Phytochemical Analysis of Flavonoids and Tannins from Ethanol Extract of Keji Beling (Strobilanthes crispus) Using UV-Vis Spectrophotometry Method

Ayu Safitri, Panji Wahlanto
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Abstract

Keji Beling (Strobilanthes crispus BI.) is a plant that has been used by the community as a traditional medicine for kidney stones, diabetes mellitus, ulcers and as a laxative. Keji beling contains many beneficial substances such as flafonoids and tannins. This research aims to determine the content of flavonoid and tannin phytochemical compounds and to determine length Maximum waves in flavonoid and tannin compounds in keji beling leaf extract (Strobilanthes crispus BI.). Keji beling leaves were extracted using the maceration method with 70% ethanol solvent. Secondary metabolite content was identified qualitatively using a phytochemical screening test. Flavonoids were tested with concentrated MgSO4 and HCl reagents and tannins were tested with FeCl3 reagent. Qualitative analysis using the UV-Vis Spectrophotometry method. Results Research shows that the phytochemical screening test on keji beling leaf extract (Strobilanthes crispus BI.) is positive for containing flavonoids and tannins, indicated by a color change in the phytochemical screening test. Qualitative analysis results appear at a maximum wavelength  of 465 nm for flavonoids, and for tannin compounds at a maximum wavelength  of 765 nm.
用紫外可见分光光度法分析克氏贝林(Strobilanthes crispus)乙醇提取物中的类黄酮和单宁的植物化学成分
Keji Beling(Strobilanthes crispus BI.)是一种被社区用作治疗肾结石、糖尿病、溃疡和泻药的传统药物的植物。Keji beling 含有许多有益物质,如呋喃类化合物和单宁酸。本研究旨在确定黄酮类和单宁类植物化学物质的含量,并确定枸杞叶提取物(Strobilanthes crispus BI.)中黄酮类和单宁类化合物的最大波长。用 70% 的乙醇溶剂浸泡法提取枸杞叶。采用植物化学筛选试验对次生代谢物含量进行定性鉴定。黄酮类化合物用浓 MgSO4 和 HCl 试剂进行测试,单宁酸用 FeCl3 试剂进行测试。采用紫外可见分光光度法进行定性分析。研究结果表明,Keji Beling 叶提取物(Strobilanthes crispus BI.)的植物化学筛选测试结果呈阳性,含有黄酮类化合物和单宁酸,植物化学筛选测试中的颜色变化表明了这一点。定性分析结果显示,黄酮类化合物的最大波长为 465 纳米,单宁化合物的最大波长为 765 纳米。
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