Sanjeev Khanth P E , Akhilesh Mishra , Shramana Mandal , Shalini Chawla , Bhupinder Singh Kalra
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引用次数: 0
Abstract
Background
Isoniazid (INH) and Rifampicin (RIF) are the common drugs causing hepatitis in patients undergoing Antitubercular therapy (ATT). This often results in discontinuation of the therapy or change in the treatment regimen. Free radical injury by drug metabolites has been postulated as cause of liver damage. The herbal extracts of Phyllanthus niruri and Andrographis paniculata possess antioxidant activity.
Methods
Hepatotoxicity was induced in Sprague Dawley rats by administering Isoniazid (100 mg/kg, po) and Rifampicin (100 mg/kg, po) combination for 14 days. Each group of rats were simultaneously treated with P. niruri (125 mg/kg, po), A. paniculata (125 mg/kg, po) and in combination of both for 14 days. Assessment of hepatotoxicity was done by evaluating serum total bilirubin, serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), liver superoxide dismutase (SOD), liver catalase levels, and histopathological examination of liver.
Results
There was a significant reduction in the total bilirubin and SGOT levels in the groups treated with P.niruri, A.paniculata and combination of P.niruri and A.paniculata when compared with the INH and RMP treated groups (P<; 0.0001, P = 0.03, P < 0.0001) respectively. The liver SOD and Catalase enzymes were significantly deranged in the group treated with INH and RMP compared to the control animals (P < 0.0001). However, the SOD and Catalase levels were significantly elevated in the groups treated with these protective agents (P < 0.0001) when compared with INH and RMP group. Histopathological examination revealed that in the groups treated with INH and RMP, grades 1 & 2 necrosis was observed than those animals of the normal control group (p = 0.002). However, treatment with P.niruri and A.paniculata extracts showed no signs of necrosis on comparing with the INH and RMP group (p = 0.002).
Conclusion
Extracts of P. niruri and A. paniculata both in monotherapy and combination has hepatoprotective action against the hepatotoxicity induced by Isoniazid and Rifampicin.
期刊介绍:
Indian Journal of Tuberculosis (IJTB) is an international peer-reviewed journal devoted to the specialty of tuberculosis and lung diseases and is published quarterly. IJTB publishes research on clinical, epidemiological, public health and social aspects of tuberculosis. The journal accepts original research articles, viewpoints, review articles, success stories, interesting case series and case reports on patients suffering from pulmonary, extra-pulmonary tuberculosis as well as other respiratory diseases, Radiology Forum, Short Communications, Book Reviews, abstracts, letters to the editor, editorials on topics of current interest etc. The articles published in IJTB are a key source of information on research in tuberculosis. The journal is indexed in Medline