Hepatoprotective potential of Phyllanthus niruri and Andrographis paniculata in isoniazid-rifampicin induced hepatotoxicity in rats

Q3 Medicine
Sanjeev Khanth P E , Akhilesh Mishra , Shramana Mandal , Shalini Chawla , Bhupinder Singh Kalra
{"title":"Hepatoprotective potential of Phyllanthus niruri and Andrographis paniculata in isoniazid-rifampicin induced hepatotoxicity in rats","authors":"Sanjeev Khanth P E ,&nbsp;Akhilesh Mishra ,&nbsp;Shramana Mandal ,&nbsp;Shalini Chawla ,&nbsp;Bhupinder Singh Kalra","doi":"10.1016/j.ijtb.2023.12.009","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div><span><span>Isoniazid (INH) and Rifampicin (RIF) are the common drugs causing hepatitis in patients undergoing Antitubercular therapy (ATT). This often results in discontinuation of the therapy or change in the treatment regimen. </span>Free radical<span><span> injury by </span>drug metabolites has been postulated as cause of liver damage. The herbal extracts of </span></span><span><em>Phyllanthus niruri</em></span> and <span><em>Andrographis paniculata</em></span><span> possess antioxidant activity.</span></div></div><div><h3>Methods</h3><div><span>Hepatotoxicity was induced in Sprague Dawley rats by administering Isoniazid (100 mg/kg, po) and Rifampicin (100 mg/kg, po) combination for 14 days. Each group of rats were simultaneously treated with </span><em>P. niruri</em> (125 mg/kg, po), <em>A. paniculata</em><span><span> (125 mg/kg, po) and in combination of both for 14 days. Assessment of hepatotoxicity was done by evaluating serum total bilirubin<span><span>, serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), liver </span>superoxide dismutase (SOD), liver </span></span>catalase levels, and histopathological examination of liver.</span></div></div><div><h3>Results</h3><div>There was a significant reduction in the total bilirubin and SGOT levels in the groups treated with <em>P.niruri, A.paniculata</em> and combination of <em>P.niruri and A.paniculata</em> when compared with the INH and RMP treated groups (P&lt;; 0.0001, P = 0.03, P &lt; 0.0001) respectively. The liver SOD and Catalase enzymes were significantly deranged in the group treated with INH and RMP compared to the control animals (P &lt; 0.0001). However, the SOD and Catalase levels were significantly elevated in the groups treated with these protective agents (P &lt; 0.0001) when compared with INH and RMP group. Histopathological examination revealed that in the groups treated with INH and RMP, grades 1 &amp; 2 necrosis was observed than those animals of the normal control group (p = 0.002). However, treatment with <em>P.niruri</em> and <em>A.paniculata</em> extracts showed no signs of necrosis on comparing with the INH and RMP group (p = 0.002).</div></div><div><h3>Conclusion</h3><div>Extracts of <em>P. niruri</em> and <em>A. paniculata</em><span> both in monotherapy and combination has hepatoprotective action against the hepatotoxicity induced by Isoniazid and Rifampicin.</span></div></div>","PeriodicalId":39346,"journal":{"name":"Indian Journal of Tuberculosis","volume":"72 2","pages":"Pages 189-193"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Tuberculosis","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0019570723002263","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

Abstract

Background

Isoniazid (INH) and Rifampicin (RIF) are the common drugs causing hepatitis in patients undergoing Antitubercular therapy (ATT). This often results in discontinuation of the therapy or change in the treatment regimen. Free radical injury by drug metabolites has been postulated as cause of liver damage. The herbal extracts of Phyllanthus niruri and Andrographis paniculata possess antioxidant activity.

Methods

Hepatotoxicity was induced in Sprague Dawley rats by administering Isoniazid (100 mg/kg, po) and Rifampicin (100 mg/kg, po) combination for 14 days. Each group of rats were simultaneously treated with P. niruri (125 mg/kg, po), A. paniculata (125 mg/kg, po) and in combination of both for 14 days. Assessment of hepatotoxicity was done by evaluating serum total bilirubin, serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), liver superoxide dismutase (SOD), liver catalase levels, and histopathological examination of liver.

Results

There was a significant reduction in the total bilirubin and SGOT levels in the groups treated with P.niruri, A.paniculata and combination of P.niruri and A.paniculata when compared with the INH and RMP treated groups (P<; 0.0001, P = 0.03, P < 0.0001) respectively. The liver SOD and Catalase enzymes were significantly deranged in the group treated with INH and RMP compared to the control animals (P < 0.0001). However, the SOD and Catalase levels were significantly elevated in the groups treated with these protective agents (P < 0.0001) when compared with INH and RMP group. Histopathological examination revealed that in the groups treated with INH and RMP, grades 1 & 2 necrosis was observed than those animals of the normal control group (p = 0.002). However, treatment with P.niruri and A.paniculata extracts showed no signs of necrosis on comparing with the INH and RMP group (p = 0.002).

Conclusion

Extracts of P. niruri and A. paniculata both in monotherapy and combination has hepatoprotective action against the hepatotoxicity induced by Isoniazid and Rifampicin.

Abstract Image

穿心莲和穿心莲对异烟肼-利福平诱导的大鼠肝毒性的保护潜力
背景二烟肼(INH)和利福平(RIF)是接受抗结核治疗(ATT)的患者引起肝炎的常见药物。这通常导致停止治疗或改变治疗方案。由药物代谢物引起的自由基损伤被认为是肝损伤的原因。余甘子和穿心莲提取物具有抗氧化活性。方法异烟肼(100 mg/kg, po)与利福平(100 mg/kg, po)联合给药14 d,对大鼠产生细胞毒性。每组大鼠同时给予尼氏弓形虫(125 mg/kg, po)、金针菇(125 mg/kg, po)或两者联合治疗,疗程14 d。通过血清总胆红素、血清天冬氨酸转氨酶(AST)、血清丙氨酸转氨酶(ALT)、肝脏超氧化物歧化酶(SOD)、肝脏过氧化氢酶水平及肝脏组织病理学检查评估肝毒性。结果与INH和RMP治疗组相比,尼罗菌组、金针菇组和尼罗菌与金针菇联合治疗组总胆红素和SGOT水平显著降低(p < 0.05;0.0001, P = 0.03, P <;分别为0.0001)。与对照组相比,INH和RMP组肝脏SOD和过氧化氢酶明显紊乱(P <;0.0001)。然而,这些保护剂处理组的SOD和过氧化氢酶水平显著升高(P <;0.0001),与INH和RMP组比较。组织病理学检查显示,在INH和RMP治疗组,1级和amp;与正常对照组相比,出现2个坏死灶(p = 0.002)。然而,与INH和RMP组相比,尼鲁氏和荆芥提取物组未出现坏死迹象(p = 0.002)。结论对异烟肼和利福平所致的肝毒性,牛耳和金银花提取物单独或联合治疗均有肝保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Indian Journal of Tuberculosis
Indian Journal of Tuberculosis Medicine-Infectious Diseases
CiteScore
2.80
自引率
0.00%
发文量
103
期刊介绍: Indian Journal of Tuberculosis (IJTB) is an international peer-reviewed journal devoted to the specialty of tuberculosis and lung diseases and is published quarterly. IJTB publishes research on clinical, epidemiological, public health and social aspects of tuberculosis. The journal accepts original research articles, viewpoints, review articles, success stories, interesting case series and case reports on patients suffering from pulmonary, extra-pulmonary tuberculosis as well as other respiratory diseases, Radiology Forum, Short Communications, Book Reviews, abstracts, letters to the editor, editorials on topics of current interest etc. The articles published in IJTB are a key source of information on research in tuberculosis. The journal is indexed in Medline
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信