The chemical composition of Diwu YangGan capsule and its potential inhibitory roles on hepatocellular carcinoma by microarray-based transcriptomics

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Qingxin Shi , Jiangcheng He , Guangya Chen , Jinlin Xu , Zhaoxiang Zeng , Xueyan Zhao , Binbin Zhao , Xiang Gao , Zhihua Ye , Mingzhong Xiao , Hanmin Li
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Abstract

The Traditional Chinese Medicine compound preparation known as Diwu Yanggan capsule (DWYG) can effectively hinder the onset and progression of hepatocellular carcinoma (HCC), which is recognized worldwide as a significant contributor to fatalities associated with cancer. Nevertheless, the precise mechanisms implicated have remained ambiguous. In present study, the model of HCC was set up by the 2-acetylaminofluorene (2-AAF)/partial hepatectomy (PH) in rats. To confirm the differentially expressed genes (DEGs) identified in the microarray analysis, real-time quantitative reverse transcription PCR (qRT-PCR) was conducted. In the meantime, the liquid chromatography-quadrupole time of flight mass spectrometry (LC-QTOF-MS/MS) was employed to characterize the component profile of DWYG. Consequently, the DWYG treatment exhibited the ability to reverse 51 variation genes induced by 2-AAF/PH. Additionally, there was an overlap of 54 variation genes between the normal and model groups. Upon conducting RT-qPCR analysis, it was observed that the expression levels of all genes were increased by 2-AAF/PH and subsequently reversed after DWYG treatment. Notably, the fold change of expression levels for all genes was below 0.5, with 3 genes falling below 0.25. Moreover, an investigation was conducted to determine the signaling pathway that was activated/inhibited in the HCC group and subsequently reversed in the DWYG group. Moreover, the component profile of DWYG encompassed a comprehensive compilation of 206 compounds that were identified or characterized. The findings of this study elucidated the potential alleviative mechanisms of DWYG in the context of HCC, thereby holding significant implications for its future clinical utilization and widespread adoption.

Abstract Image

基于芯片的转录组学研究地乌洋参胶囊的化学成分及其对肝细胞癌的潜在抑制作用
地乌阳干胶囊(DWYG)是一种中药复方制剂,能有效阻止肝细胞癌(HCC)的发生和发展。然而,其确切的作用机制仍不明确。本研究以 2-乙酰氨基芴(2-AAF)/部分肝切除术(PH)为大鼠建立了 HCC 模型。为了确认芯片分析中发现的差异表达基因(DEGs),研究人员进行了实时定量反转录 PCR(qRT-PCR)分析。同时,采用液相色谱-四极杆飞行时间质谱(LC-QTOF-MS/MS)分析了DWYG的成分特征。结果表明,DWYG 能够逆转 2-AAF/PH 诱导的 51 个变异基因。此外,正常组和模型组之间有 54 个变异基因重叠。在进行 RT-qPCR 分析时,观察到所有基因的表达水平都因 2-AAF/PH 而增加,并在 DWYG 处理后逆转。值得注意的是,所有基因表达水平的折合变化均低于 0.5,其中 3 个基因低于 0.25。此外,研究还确定了在 HCC 组中被激活/抑制,随后在 DWYG 组中被逆转的信号通路。此外,DWYG 的成分概况包含了已鉴定或表征的 206 种化合物的综合汇编。本研究的结果阐明了 DWYG 对 HCC 的潜在缓解机制,从而对其未来的临床应用和广泛采用具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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