INFLUENCE OF PHYTOSTIMULATORS ON PHENOLIC METABOLISM OF VINE CALLUS CULTURE

Abstract

The development of culture media composi-tions to improve the efficiency of grape prop-agation in in vitro culture is an important task. At the same time, the primary assessment of the impact of culture media composition on grapes is complicated by a high level of varie-ty specificity, the need for plant transplanta-tion, and the release of a large amount of tox-ic phenolic compounds into the culture media, resulting in plant death. Thus, the possibility of testing the effect of certain additives on the survival of in vitro plants on callus cul-tures as a simpler model object can signifi-cantly increase the efficiency of selection of culture media components. The article consid-ers the influence of various physiologically ac-tive substances on the changes in the synthesis of phenolic compounds in callus cultures of Krasnostop grape variety. As a result of the work, it was found that only coumaric acid from all the studied additives causes exces-sive formation of phenolic compounds in the callus culture of Krasnostop grapes. Ascorbic acid increases the growth rate of culture, re-ducing the level of oxidative processes. Me-thyl jasmonate increases phenolic metabolism, but does not lead to stress reactions, while sal-icylic acid has a general inhibitory effect.