In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions

IF 20.2 Q1 MATERIALS SCIENCE, PAPER & WOOD

Journal of Bioresources and Bioproducts Pub Date : 2024-02-01 DOI:10.1016/j.jobab.2023.12.003

Bianjing Sun , Ping Wang , Jingang Zhang , Jianbin Lin , Lingling Sun , Xiaokun Wang , Chuntao Chen , Dongping Sun

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Abstract

Bacterial cellulose (BC) hydrogel spheroid plays a significant role in diverse fields due to its spatial 3D structure and properties. In the present work, a series of BC spheroids with controllable size and shape was obtained via an in situ biosynthesis. Crucial factors for fabricating BC spheroid including inoculum concentration of 1.35 × 10³ CFU/mL, shaking speeds at 100 r/min, and 48–96 h incubation time during the biosynthetic process, were comprehensively established. An operable mechanism model for tuning the size of BC spheroids from 0.4 to 5.0 mm was proposed with a fresh feeding medium strategy of dynamic culture. The resulting BC spheroids exhibit an interactive 3D network of nanofibers, a crystallinity index of 72.3 %, a specific surface area of 91.2 m²/g, and good cytocompatibility. This study reinforces the understanding of BC spheroid formation and explores new horizons for the design of BC spheroids-derived functional matrix materials for medical care.

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原位生物合成尺寸可调的细菌纤维素水凝胶球体

细菌纤维素（BC）水凝胶球因其空间三维结构和特性而在多个领域发挥着重要作用。在本研究中，通过原位生物合成技术获得了一系列大小和形状可控的细菌纤维素球体。该研究全面确定了生物合成过程中接种物浓度为 1.35 × 103 CFU/mL、振荡速度为 100 r/min、培养时间为 48-96 h 等制造 BC 球形体的关键因素。通过动态培养的新鲜饲养培养基策略，提出了一个可操作的机制模型，用于调节 BC 球体的大小（从 0.4 毫米到 5.0 毫米）。所制备的 BC 球体呈现出交互式三维纳米纤维网络，结晶度指数为 72.3%，比表面积为 91.2 m2/g，并具有良好的细胞相容性。这项研究加深了人们对碱性纤维球形成的理解，为设计用于医疗保健的碱性纤维球衍生功能基质材料开辟了新天地。

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