MORPHOLOGICAL PATTERN OF BONE TISSUE DURING IMPLANTATION OF DISPERSED BIOCOMPOSITE BASED ON WOLLASTONITE

Abstract

The purpose of the study is to analyze the structure of the bone tissue surrounding a tooth under normal conditions, after tooth extraction and after placing wollastonite / hydroxyapatite scaffolds into the mandibular alveolus of a test animal (rabbit). Materials and Methods. Female New Zealand White rabbits (n=9), raised on a farm, were used as test animals. The animals were 12 months old, and weighed 1.7–3 kg. Surgeries were performed in a veterinary clinic in Vladivostok, under combined anesthesia. Mandibular lateral incisors were extracted in test animals. The surgery was followed by alveoli augmentation with wollastonite/hydroxyapatite scaffolds. After surgical procedures, the wounds were tightly sutured with nonabsorbable suture material (Vicryl, USA). Surgical wounds were debrided for 15 days (0.9 % NaCl solution). During first 24-hours after the operation, the animals were on a soft no chew diet. The rabbits were under dynamic observation throughout the experiment. Results. Alveoli augmentation of the extracted incisors with wollastonite / hydroxyapatite scaffolds had a positive effect on bone regeneration. It led to a significant restoration of the cortical plate thickness and preservation of the bone beams volume of the mandibular alveolar process. This is evidenced by the results of a morphological study of histologic specimen obtained from experimental subjects (mandibular alveolar process). No toxic effects on surrounding tissues were detected. Conclusion. The study allows us to confirm the bioactive qualities of the synthesized wollastonite/hydroxyapatite scaffolds and prove the lack of toxic side-effects on surrounding tissues.