Protein Hydrolysate from Waste of Catfish Fillet Processing for Snakehead Fish Feed Formulation

IF 2.3 Q2 BIOLOGY

Scientifica Pub Date : 2023-12-29 DOI:10.1155/2023/2815122

Mulyasari, Subaryono, Bagus Sediadi, Bandol Utomo, Imam Taufk, Muhamad Yamin, I. I. Kusmini, Tri Marwati

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引用次数: 0

Abstract

The negative impact of fish processing industry waste needs to be minimized, by processing it into valuable products, one of which is fish feed. The objectives of this research were to determine the optimum dose of crude extract of B. cereus RGL.1.1 enzyme in hydrolyzing protein from the waste of catfish (Pangasius hypophthalmus) fillet processing and to evaluate the effectiveness of using protein hydrolysate in snakehead fish (Channa striata) feed. There were four doses of enzyme treatment for protein hydrolysis designed in a completely randomized design, namely, 0, 4, 6, and 8% (v/w) with three repetitions. Furthermore, to assess the quality of protein hydrolysate, an analysis of soluble protein level, hydrolysis degree, amino acid content, fatty acid content, and digestibility was carried out. The percentage of protein hydrolysate applied in the feed formula was 0, 15, 30, and 45% (v/w), which was designed in a completely randomized design with three replications. Absolute weight growth, specific growth rate, protein efficiency ratio, feed efficiency, and snakehead fish survival were measured to evaluate the effects of the feed formula. Results showed that the crude extract of B. cereus RGL.1.1 enzymes at a concentration of 6% (v/w) enhanced the availability of soluble proteins, amino acids, fatty acids, and feed digestibility. Protein hydrolysate application in snakehead fish feed formula up to 45% (v/w) can improve the growth performance (8.03%), protein efficiency ratio (25.66%), and feed efficiency (23.41%).

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利用鲶鱼片加工废料水解蛋白配制蛇头鱼饲料

鱼类加工业废弃物的负面影响需要通过将其加工成有价值的产品（鱼饲料就是其中之一）来最小化。本研究的目的是确定 B. cereus RGL.1.1 酶粗提取物水解鲶鱼（Pangasius hypophthalmus）鱼片加工废料中蛋白质的最佳剂量，并评估在乌鳢（Channa striata）饲料中使用蛋白质水解物的效果。采用完全随机设计法设计了四种蛋白水解酶处理剂量，即 0%、4%、6% 和 8%（v/w），重复三次。此外，为了评估水解蛋白的质量，还对可溶性蛋白含量、水解度、氨基酸含量、脂肪酸含量和消化率进行了分析。饲料配方中蛋白水解物的比例为 0、15、30 和 45%（v/w），采用完全随机设计，三次重复。测定了绝对重量增长率、特定生长率、蛋白质效率比、饲料效率和乌鳢存活率，以评估饲料配方的效果。结果表明，6%（体积分数）浓度的蜡样芽孢杆菌 RGL.1.1 酶粗提取物提高了可溶性蛋白质、氨基酸、脂肪酸的可利用性和饲料消化率。蛋白质水解物在乌鳢饲料配方中的应用浓度达到 45%（体积分数）时，可提高乌鳢的生长性能（8.03%）、蛋白质效率比（25.66%）和饲料效率（23.41%）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientifica BIOLOGY-

CiteScore

6.70

自引率

0.00%

发文量

审稿时长

21 weeks

期刊介绍： Scientifica is a peer-reviewed, Open Access journal that publishes research articles, review articles, and clinical studies covering a wide range of subjects in the life sciences, environmental sciences, health sciences, and medicine. The journal is divided into the 65 subject areas.