Investigation of Antimicrobial Susceptibility Patterns and blaVIM -metallo-β-lactamase Gene in Clinical Samples of Klebsiella pneumoniae

University of Thi-Qar Journal of Science Pub Date : 2023-12-30 DOI:10.32792/utq/utjsci/v10i2.1149

Asaad Alkhafaji, Neda Soleimani, Hind Mousa

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Abstract

Multidrug resistance is a widespread issue that plays an important role in disease outcome. This study was designed to isolate Klebsiella pneumonia in different clinical specimens and detected their Antibiotic resistance profile. A total of 319 samples were collected from various clinical specimens for both genders and different ages. The samples were streaked on the blood and MacConkey agars. The bacterial growth identified using biochemical tests and Vitek®2 systems to confirm it. Also, the Vitek®2 system was used to detect the antibiotic sensitivity. Out of 319 clinical samples, K. pneumonia was identified in 67 (21%) cases. The highest isolation rate was in urine 25(37.3%), followed by sputum 13(19.4%), and the least isolation was in CSF with one isolate (1.5%). The results revealed that K. pneumonia isolates were multi-drug resistant pathogens (MDR) with high resistance to Ampicillin (97%) and 85% for piperacillin. The PCR results revealed blaVIM- genes frequency was 20 (30%). K. pneumonia is one of the bacteria that cause urinary tract infections, and it is a widespread multidrug-resistant pathogen, and blaVIM- producing K. pneumoniae are found in clinical samples at Thi-Qar hospitals. Therefore, monitoring the administration of antibiotics and their rational use is necessary to reduce antimicrobial resistance and treatment failure.

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肺炎克雷伯菌临床样本的抗菌药敏感性模式和 blaVIM -金属-β-内酰胺酶基因研究

多重耐药性是一个普遍存在的问题，对疾病的治疗结果起着重要作用。本研究旨在分离不同临床标本中的肺炎克雷伯氏菌，并检测其抗生素耐药性情况。研究人员从不同的临床标本中收集了 319 份样本，这些样本来自不同的性别和年龄。这些样本分别在血琼脂和麦康凯琼脂上做条痕培养。使用生化测试和 Vitek®2 系统对细菌生长情况进行确认。此外，Vitek®2 系统还用于检测抗生素敏感性。在 319 份临床样本中，有 67 例（21%）鉴定出肺炎克氏菌。分离率最高的是尿液，有 25 例（37.3%），其次是痰液，有 13 例（19.4%），分离率最低的是脑脊液，有 1 例（1.5%）。结果显示，肺炎克雷伯菌属多重耐药病原体（MDR），对氨苄西林耐药率高（97%），对哌拉西林耐药率高（85%）。PCR 结果显示 blaVIM- 基因频率为 20（30%）。肺炎双球菌是导致尿路感染的细菌之一，是一种广泛存在的多重耐药病原体，在济喀医院的临床样本中发现了产生 blaVIM- 的肺炎双球菌。因此，有必要监测抗生素的使用情况并合理使用抗生素，以减少抗生素耐药性和治疗失败。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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University of Thi-Qar Journal of Science

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