Genetic analysis of fatty acids, oil and protein percent in maize (zea mays l.) Using partial diallel mating design

S. M. Sheikh Abdulla, Dana Azad Abulkhaleq, S. Towfiq
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Abstract

In this study, the hybridizations were done using partial diallel mating design in spring season 2020 at Qlyasan location to complete the procedure of this experiment, which conducted during spring season 2021 at two different locations in Sulaimani Governorate, Qlyasan and Kanipanka Using randomized Complete Block Design (RCBD) with three replication. Eight pure lines of maize and their partial cross-hybrids were used by adopting a sample of three crosses for each line (MGW 16, NADH 102, NADH 704, NADH 706, NADH 52, ZY 52, NADH 905, MSI 4279). All genotypes (8 parents + 12 F1s hybrids) were prepared to chemical analysis for estimating oil%, protein% and fatty acids (Palmitic, Stearic, Linolic, Oleic, Linolenic and Oil). The results summarized as follow: The analysis of variance revealed that the mean square due to crosses and gca was highly significant for all studied traits, while for sca it was highly significant for most traits at both locations. The mean square due to the parents were highly significant for most studied traits. The cross NADH 52 × MSI 4279 and parent MSI 4279 showed gave highest means values for all traits. positive heterosis% for all traits was observed. The cross NADH 52 × MSI 4279 showed the highest heterosis% for all traits at both locations. The contribution of additive and non-additive gene effect was important in the inheritance of these traits at Qlyasan location, while at the Kani panka location the contribution of additive gene effect was more than non-additive gene effect for all traits. Highly significant correlation was recorded between each pair of fatty acids at both locations, but kernel yield recorded significant correlation with linolic and oleic acids at Kanipanka location only.
玉米(zea mays l.)脂肪酸、油脂和蛋白质百分比的遗传分析--采用部分双倍交配设计
本研究于 2020 年春季在苏莱曼尼省的两个不同地点(Qlyasan 和 Kanipanka)采用随机完全区组设计(RCBD)进行杂交。每个品系采用三个杂交种(MGW 16、NADH 102、NADH 704、NADH 706、NADH 52、ZY 52、NADH 905、MSI 4279),使用了八个玉米纯系及其部分杂交种。对所有基因型(8 个亲本 + 12 个 F1s 杂交种)进行了化学分析,以估算油脂率、蛋白质率和脂肪酸(棕榈酸、硬脂酸、亚麻酸、油酸、亚麻酸和油)。结果总结如下:方差分析显示,杂交和 gca 的均方差对所有研究性状都非常显著,而 sca 的均方差对两地的大多数性状都非常显著。亲本的均方差对大多数研究性状都非常显著。杂交种 NADH 52 × MSI 4279 和亲本 MSI 4279 在所有性状上都表现出最高的平均值。杂交种 NADH 52 × MSI 4279 在两个地点的所有性状上都表现出最高的异质性。在 Qlyasan 地点,加性和非加性基因效应对这些性状的遗传有重要影响,而在 Kani panka 地点,加性基因效应对所有性状的影响均大于非加性基因效应。两地的每对脂肪酸之间都存在高度显著的相关性,但只有卡尼潘卡地区的果仁产量与亚麻酸和油酸存在显著的相关性。
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