Jinwei Liu, Weichang Xu, Feng Yan, Xinqi Liu, Yuan Wang
{"title":"Cleaning Process of Diosgenin from Dioscorea nipponica","authors":"Jinwei Liu, Weichang Xu, Feng Yan, Xinqi Liu, Yuan Wang","doi":"10.1166/jbmb.2024.2335","DOIUrl":null,"url":null,"abstract":"This investigation employed HPLC and LC-MS techniques to elucidate the enzymolysis and acid hydrolysis mechanisms of diosgenin obtained through a cleaning process. The findings revealed that the enzymolysis led to the cleavage and subsequent recombination of the glycosidic bond at the C-26 position of protodioscin, resulting in the formation of dioscin present in the enzymatic hydrolysis filter residue. Leveraging this observation, a streamlined and eco-friendly method for diosgenin extraction was devised. Incorporating the Box-Behnken response surface methodology alongside wastewater assessment, the optimal parameters for the cleaning process were established: a sulfuric acid concentration of 3 mol · L−1, a solid–liquid ratio of 1:10, an acid hydrolysis temperature of 100 °C, and an acid hydrolysis duration of 3 h. Under these parameters, the yield and purity of diosgenin were 31.07±0.56% and 72.30±0.24% respectively. When benchmarked against the direct acid hydrolysis approach, there was an increase of 133.08% in diosgenin yield, 44.08% enhancement in diosgenin purity, 50% reduction in wastewater generation and acid utilization, and an 83.57% decrease in wastewater’s chemical oxygen demand (COD). This optimized cleaning process is viable for large-scale production and offers a sustainable method for diosgenin production.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":"139 15","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biobased Materials and Bioenergy","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbmb.2024.2335","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
This investigation employed HPLC and LC-MS techniques to elucidate the enzymolysis and acid hydrolysis mechanisms of diosgenin obtained through a cleaning process. The findings revealed that the enzymolysis led to the cleavage and subsequent recombination of the glycosidic bond at the C-26 position of protodioscin, resulting in the formation of dioscin present in the enzymatic hydrolysis filter residue. Leveraging this observation, a streamlined and eco-friendly method for diosgenin extraction was devised. Incorporating the Box-Behnken response surface methodology alongside wastewater assessment, the optimal parameters for the cleaning process were established: a sulfuric acid concentration of 3 mol · L−1, a solid–liquid ratio of 1:10, an acid hydrolysis temperature of 100 °C, and an acid hydrolysis duration of 3 h. Under these parameters, the yield and purity of diosgenin were 31.07±0.56% and 72.30±0.24% respectively. When benchmarked against the direct acid hydrolysis approach, there was an increase of 133.08% in diosgenin yield, 44.08% enhancement in diosgenin purity, 50% reduction in wastewater generation and acid utilization, and an 83.57% decrease in wastewater’s chemical oxygen demand (COD). This optimized cleaning process is viable for large-scale production and offers a sustainable method for diosgenin production.