The diagnostic value of GICA used for intraoperative lymph node FNA-Tg measurement to evaluate thyroid cancer metastases.

IF 3.5 2区医学 Q2 ENDOCRINOLOGY & METABOLISM

European Thyroid Journal Pub Date : 2024-01-29 Print Date: 2024-02-01 DOI:10.1530/ETJ-23-0182

Shaodong Hou, Yiceng Sun, Zeyu Yang, Mi Tang, Tingjie Yin, Cong Shao, Cunye Yan, Linlong Mo, Yuquan Yuan, Supeng Yin, Fan Zhang

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引用次数: 0

Abstract

Objective: It is crucial to diagnose lymph node (LN) metastases (LNM) before or during thyroid carcinoma surgery. Measurement of thyroglobulin (Tg) in the fine needle aspirate washout (FNA-Tg) is useful to assist in the diagnosis of LNM for papillary thyroid carcinoma (PTC). This study aimed to assess the diagnostic performance of a new technique based on a colloidal gold-based immunochromatographic assay (GICA) for intraoperative FNA-Tg in diagnosing LNM.

Clinical trial information: This study is registered with chictr.org.cn, ID: ChiCTR2200063561 (registered 11 September, 2022).

Methods: This prospective study enrolled 51 PTC patients who underwent cervical LN dissection. A total of 150 LNs dissected from the central and lateral compartments were evaluated by FNA-Tg-GICA at three different time points and compared with frozen sections and the conventional Tg measurement method electrochemiluminescence immunoassay (ECLIA). Receiver operating characteristic curve (ROC) and area under the curve (AUC), cutoff value to discriminate benign and malignant LNs, sensitivity, specificity, and accuracy were provided.

Results: The cutoff value of FNA-Tg to predict LNM was 110.83 ng/mL for ECLIA and 13.19 ng/mL, 38.69 ng/mL, and 77.17 ng/mL for GICA at 3, 10, and 15 min, respectively. There was no significant difference between the AUCs of GICA at different time points compared to using ECLIA and frozen sections. Besides, the diagnostic performance of GICA and ECLIA showed no significant difference in evaluating LNM from central and lateral compartments or between the TgAb-positive subgroup and TgAb-negative subgroup.

Conclusion: GICA is a promising method for intraoperative FNA-Tg measurement and has high value in predicting LNM. It may be a novel alternative or supplementary method to frozen section or ECLIA.

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用于术中淋巴结 FNA-Tg 测量的 GICA 对评估甲状腺癌转移的诊断价值

目的：在甲状腺癌手术前或手术中诊断淋巴结转移（LNM）至关重要。测定细针穿刺冲洗液（FNA-Tg）中的甲状腺球蛋白（Tg）有助于辅助诊断甲状腺乳头状癌（PTC）的淋巴结转移。本研究旨在评估一种基于胶体金免疫层析（GICA）的新技术在术中FNA-Tg诊断LNM中的诊断性能：这项前瞻性研究共纳入51例接受颈部LN切除术的PTC患者。在三个不同的时间点，通过 FNA-Tg-GICA 对从中央区和外侧区切除的 150 个 LN 进行了评估，并与冰冻切片和传统的 Tg 测量方法电化学发光免疫测定（ECLIA）进行了比较。结果显示了接收者操作特征曲线（ROC）和曲线下面积（AUC）、区分良性和恶性 LN 的临界值、灵敏度、特异性和准确性：ECLIA预测LNM的FNA-Tg临界值为110.83纳克/毫升，GICA在3分钟、10分钟和15分钟预测LNM的临界值分别为13.19纳克/毫升、38.69纳克/毫升和77.17纳克/毫升。与使用 ELICA 和冰冻切片相比，GICA 在不同时间点的 AUC 无明显差异。此外，GICA和ECLIA在评估中央区和外侧区的LNM时，以及在TgAb阳性亚组和TgAb阴性亚组之间，诊断性能均无明显差异：GICA 是一种很有前途的术中 FNA-Tg 测量方法，在预测 LNM 方面具有很高的价值。它可能是冰冻切片或 ECLIA 的新型替代或补充方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Thyroid Journal Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

6.70

自引率

2.10%

发文量

156

期刊介绍： The ''European Thyroid Journal'' publishes papers reporting original research in basic, translational and clinical thyroidology. Original contributions cover all aspects of the field, from molecular and cellular biology to immunology and biochemistry, from physiology to pathology, and from pediatric to adult thyroid diseases with a special focus on thyroid cancer. Readers also benefit from reviews by noted experts, which highlight especially active areas of current research. The journal will further publish formal guidelines in the field, produced and endorsed by the European Thyroid Association.