MicroRNA-193a Promotes Apoptosis in Retinal Neuronal Cells in Early-Stage Diabetic (DM) Rat via Wilms' Tumor Gene 1.

IF 1.1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Annals of clinical and laboratory science Pub Date : 2024-01-04

Juan Yu, Kehai Che, Chong Gao, Xuemei Pu, Lijun Ren

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引用次数: 0

Abstract

Objective: This study aimed to investigate the role and mechanism of microRNA (miR)-193a in promoting apoptosis of retinal neuronal cells in early diabetic (DM) rats.

Methods: Seventy-two male SD-grade rats were selected to establish a DM model by intraperitoneal injection of streptozotocin (STZ), and randomly divided into a control group (blank control group), a DM group (diabetic model group), a DM+miR-NC inhibitor group (miR-193a inhibition negative control group), a DM+miR-193a inhibitor group (miR-193a inhibitor group), DM+miR-NC mimic group (miR-193a overexpression negative control group), DM+miR-193a mimic group (miR-193a overexpression group), with12 rats in each group.

Results: The miR-193a expression, apoptosis rate, and Bax, Caspase3, and Caspase9 protein expression levels were elevated, and Bcl-2 protein expression was decreased in the retinal tissues of DM rats and high glucose-induced rat retinal neuronal cells, while miR-193a inhibitors reversed these processes. These dual luciferase reporter assay showed that WT1CDS, and WT1Mut were lower in the miR-193a group than in the miR-NC group (P<0.05); WT1 protein expression was reduced in the retinal tissues of DM rat and high glucose-induced rat retinal neuronal cells, and miR-193a inhibitors increased WT1 protein expression. Compared with cells co-transfected with miR-193a and WT1vector, miR-193a and WT1 cotransfection inhibited high glucose-induced apoptosis in retinal neuronal cells and regulated apoptotic protein expression. miR-193a was highly expressed and WT1 was lowly expressed in retinal tissues of DM rats and high glucose-induced rat retinal neuronal cells.

Conclusion: miR-193a could inhibit early retinal neuronal cell apoptosis in DM rats by targeting and negatively regulating WT1 expression.

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MicroRNA-193a 通过 Wilms' Tumor Gene 1 促进早期糖尿病 (DM) 大鼠视网膜神经元细胞的凋亡

研究目的本研究旨在探讨microRNA（miR）-193a在促进早期糖尿病（DM）大鼠视网膜神经细胞凋亡中的作用和机制：方法：选取72只雄性SD级大鼠腹腔注射链脲佐菌素（STZ）建立DM模型，随机分为对照组（空白对照组）、DM组（糖尿病模型组）和miR-193a抑制组（DM+miR-NC抑制组）、DM+miR-NC抑制剂组（miR-193a抑制阴性对照组）、DM+miR-193a抑制剂组（miR-193a抑制剂组）、DM+miR-NC模拟组（miR-193a过表达阴性对照组）、DM+miR-193a模拟组（miR-193a过表达组），每组12只。结果在DM大鼠视网膜组织和高糖诱导的大鼠视网膜神经细胞中，miR-193a表达、凋亡率、Bax、Caspase3和Caspase9蛋白表达水平升高，Bcl-2蛋白表达降低，而miR-193a抑制剂可逆转这些过程。这些双荧光素酶报告实验表明，miR-193a组的WT1CDS和WT1Mut低于miR-NC组（结论：miR-193a可通过靶向和负调控WT1的表达来抑制DM大鼠早期视网膜神经细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.