DNA origami-enhanced binding of aptamers to Staphylococcus aureus cells

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X Pub Date : 2024-01-05 DOI:10.1016/j.biosx.2023.100436

Yasaman Ahmadi , Trishant R. Umrekar , Natalie Mutter , Morgan Beeby , Ivan Barišić

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引用次数: 0

Abstract

The combination of DNA origami nanostructures and aptamers provides a powerful technology for diagnostic assays. Here, we functionalized a DNA origami nanostructure with a Protein-A binding aptamer to target Staphylococcus aureus bacterial cells. Using an enzyme-linked oligonucleotide assay (ELONA), we semi-quantitatively analyzed and compared the interaction of the aptamer and aptamer-modified DNA origamis with Staphylococcus aureus bacterial isolates. The results showed that aptamer-functionalized DNA nanostructures bind with five times higher affinity (K_D: 34 ± 5 nM) compared to the aptamer alone (K_D: 160 ± 9 nM). Visualising the interaction of bacterial cells and nanostructures with electron cryotomography further confirmed the aptamer-mediated specific interaction of DNA nanostructures with bacterial cells.

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DNA 起源增强的适配体与金黄色葡萄球菌细胞的结合

DNA 折纸纳米结构与适配体的结合为诊断分析提供了一种强大的技术。在这里，我们将 DNA 折纸纳米结构与蛋白-A 结合适配体功能化，以金黄色葡萄球菌细菌细胞为靶标。我们使用酶联寡核苷酸测定法（ELONA）半定量地分析和比较了适配体和适配体修饰的DNA折纸与金黄色葡萄球菌细菌分离物的相互作用。结果表明，与单独的适配体（KD：160 ± 9 nM）相比，适配体功能化 DNA 纳米结构的结合亲和力（KD：34 ± 5 nM）高出五倍。利用电子冷冻成像技术观察细菌细胞与纳米结构的相互作用，进一步证实了由适配体介导的 DNA 纳米结构与细菌细胞的特异性相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics: X Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

166

审稿时长

54 days

期刊介绍： Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.