Recent research progress in glycosylphosphatidylinositol-anchored protein biosynthesis, chemical/chemoenzymatic synthesis, and interaction with the cell membrane

IF 6.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zhongwu Guo , Sayan Kundu
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引用次数: 0

Abstract

Glycosylphosphatidylinositol (GPI) attachment to the C-terminus of proteins is a prevalent posttranslational modification in eukaryotic species, and GPIs help anchor proteins to the cell surface. GPI-anchored proteins (GPI-APs) play a key role in various biological events. However, GPI-APs are difficult to access and investigate. To tackle the problem, chemical and chemoenzymatic methods have been explored for the preparation of GPI-APs, as well as GPI probes that facilitate the study of GPIs on live cells. Substantial progress has also been made regarding GPI-AP biosynthesis, which is helpful for developing new synthetic methods for GPI-APs. This article reviews the recent advancements in the study of GPI-AP biosynthesis, GPI-AP synthesis, and GPI interaction with the cell membrane utilizing synthetic probes.

Abstract Image

Abstract Image

糖基磷脂酰肌醇锚定蛋白的生物合成、化学/化学合成以及与细胞膜相互作用方面的最新研究进展
蛋白质 C 端附着的糖基磷脂酰肌醇(GPI)是真核生物体内一种普遍的翻译后修饰,GPI 有助于将蛋白质锚定在细胞表面。GPI 锚定蛋白(GPI-APs)在各种生物事件中发挥着关键作用。然而,GPI-APs 难以获得和研究。为了解决这个问题,人们探索了制备 GPI-APs 的化学和化学酶方法,以及有助于研究活细胞上 GPIs 的 GPI 探针。在 GPI-AP 的生物合成方面也取得了重大进展,这有助于开发 GPI-AP 的新合成方法。本文回顾了利用合成探针研究 GPI-AP 生物合成、GPI-AP 合成以及 GPI 与细胞膜相互作用的最新进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Opinion in Chemical Biology
Current Opinion in Chemical Biology 生物-生化与分子生物学
CiteScore
13.30
自引率
1.30%
发文量
113
审稿时长
74 days
期刊介绍: COCHBI (Current Opinion in Chemical Biology) is a systematic review journal designed to offer specialists a unique and educational platform. Its goal is to help professionals stay informed about the growing volume of information in the field of Chemical Biology through systematic reviews.
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