Possible correlation between serum interleukin-8 levels and the activity of myositis in anti-NXP2 antibody-positive dermatomyositis.

IF 2.7 Q3 IMMUNOLOGY
Immunological Medicine Pub Date : 2024-06-01 Epub Date: 2024-01-04 DOI:10.1080/25785826.2023.2300553
Risa Konishi, Yuki Ichimura, Ryota Tanaka, Hanako Miyahara, Mari Okune, Masahide Miyamoto, Monami Hara, Atsushi Iwabuchi, Hidetoshi Takada, Yasuo Nakagishi, Mao Mizuta, Shuya Kaneko, Masaki Shimizu, Tomohiro Morio, Ichizo Nishino, Toshifumi Nomura, Naoko Okiyama
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引用次数: 0

Abstract

Anti-nuclear matrix protein 2 (NXP2) antibody-positive dermatomyositis (DM) is characterized by extensive and severe myositis. In this study, we evaluated which cytokines/chemokines involved with the activity of the myositis. We performed quantitative immunoassays using the MILLIPLEX® Multiplex Assays Using Luminex to evaluate serum levels of interferon-γ, interleukin (IL)-1β, IL-6, IL-8, IL-12p40, and tumor necrosis factor-α in samples collected over time from a 9-year-old female with anti-NXP2 antibody-positive DM. In our case, the serum level of IL-8 was elevated when the myositis worsened, and decreased in accordance with the improvement of myositis, suggesting that the serum IL-8 levels were correlated with the myositis activity. Serum levels of IL-8 in samples from five patients with anti-NXP2 antibody-positive DM and five patients with anti-transcriptional intermediary factor 1γ (TIF1γ) antibody-positive DM without both interstitial lung disease (ILD) and malignancy before starting treatments, along with five healthy controls, were also evaluate by an enzyme-linked immunosorbent assay. Serum IL-8 levels were significantly elevated in anti-NXP2 or anti-TIF1γ antibody-positive DM patients with myositis but not ILD, than healthy controls. It was suggested that serum levels of IL-8 correlate with the activity of myositis in DM including anti-NXP2 antibody-positive DM.

抗 NXP2 抗体阳性皮肌炎患者血清白细胞介素-8 水平与肌炎活动之间可能存在的相关性
抗核基质蛋白 2(NXP2)抗体阳性的皮肌炎(DM)以广泛和严重的肌炎为特征。在这项研究中,我们评估了哪些细胞因子/凝血因子与肌炎的活动有关。我们使用 MILLIPLEX® Multiplex Assays Using Luminex 进行了定量免疫测定,评估了从一名抗 NXP2 抗体阳性的 9 岁女性 DM 患者身上采集的样本中干扰素-γ、白细胞介素 (IL)-1β、IL-6、IL-8、IL-12p40 和肿瘤坏死因子-α 的血清水平。在我们的病例中,肌炎恶化时血清中的 IL-8 水平升高,肌炎好转时血清中的 IL-8 水平降低,这表明血清中的 IL-8 水平与肌炎活动相关。研究人员还用酶联免疫吸附试验评估了5名抗NXP2抗体阳性DM患者和5名抗转录中间因子1γ(TIF1γ)抗体阳性DM患者血清中IL-8的水平,这5名患者在开始治疗前既没有间质性肺病(ILD)也没有恶性肿瘤。与健康对照组相比,抗 NXP2 或抗 TIF1γ 抗体阳性的肌炎(非 ILD)DM 患者血清 IL-8 水平明显升高。研究表明,血清中的 IL-8 水平与 DM(包括抗 NXP2 抗体阳性的 DM)肌炎的活动性相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Immunological Medicine
Immunological Medicine Medicine-Immunology and Allergy
CiteScore
7.10
自引率
2.30%
发文量
19
审稿时长
19 weeks
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