Identification of NADPH Oxidase Genes Crucial for Rice Multiple Disease Resistance and Yield Traits

IF 4.8 1区农林科学 Q1 AGRONOMY

Rice Pub Date : 2024-01-03 DOI:10.1186/s12284-023-00678-5

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引用次数: 0

Abstract

Reactive oxygen species (ROS) act as a group of signaling molecules in rice functioning in regulation of development and stress responses. Respiratory burst oxidase homologues (Rbohs) are key enzymes in generation of ROS. However, the role of the nine Rboh family members was not fully understood in rice multiple disease resistance and yield traits. In this study, we constructed mutants of each Rboh genes and detected their requirement in rice multiple disease resistance and yield traits. Our results revealed that mutations of five Rboh genes (RbohA, RbohB, RbohE, RbohH, and RbohI) lead to compromised rice blast disease resistance in a disease nursery and lab conditions; mutations of five Rbohs (RbohA, RbohB, RbohC, RbohE, and RbohH) result in suppressed rice sheath blight resistance in a disease nursery and lab conditions; mutations of six Rbohs (RbohA, RbohB, RbohC, RbohE, RbohH and RbohI) lead to decreased rice leaf blight resistance in a paddy yard and ROS production induced by PAMPs and pathogen. Moreover, all Rboh genes participate in the regulation of rice yield traits, for all rboh mutants display one or more compromised yield traits, such as panicle number, grain number per panicle, seed setting rate, and grain weight, resulting in reduced yield per plant except rbohb and rbohf. Our results identified the Rboh family members involved in the regulation of rice resistance against multiple pathogens that caused the most serious diseases worldwide and provide theoretical supporting for breeding application of these Rbohs to coordinate rice disease resistance and yield traits.

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鉴定对水稻多种抗病性和产量性状至关重要的 NADPH 氧化酶基因

摘要活性氧（ROS）是水稻中的一组信号分子，具有调节发育和胁迫反应的功能。呼吸爆发氧化酶同源物（Rbohs）是产生 ROS 的关键酶。然而，人们对 Rboh 家族 9 个成员在水稻多种抗病性和产量性状中的作用并不完全了解。本研究构建了各 Rboh 基因的突变体，并检测了它们在水稻多种抗病性和产量性状中的需求。结果发现，在病圃和实验室条件下，5个Rboh基因（RbohA、RbohB、RbohE、RbohH和RbohI）的突变导致水稻稻瘟病抗性受损；5个Rboh基因（RbohA、RbohB、RbohC、RbohE和RbohH）的突变导致水稻在病圃和实验室条件下对鞘枯病的抗性受到抑制；6个Rboh基因（RbohA、RbohB、RbohC、RbohE、RbohH和RbohI）的突变导致水稻在稻田中对叶枯病的抗性下降以及PAMPs和病原体诱导的ROS产生。此外，所有 Rboh 基因都参与了水稻产量性状的调控，因为除 rbohb 和 rbohf 外，所有 rboh 突变体都表现出一种或多种产量性状受损，如圆锥花序数、每圆锥花序粒数、结实率和粒重，导致单株产量降低。我们的研究结果确定了参与调控水稻抗性的 Rboh 家族成员，这些成员参与调控水稻对多种病原菌的抗性，而这些病原菌是导致全球最严重病害的主要病原菌，我们的研究结果为应用这些 Rboh 来协调水稻抗病性和产量性状的育种提供了理论支持。

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来源期刊

Rice AGRONOMY-

CiteScore

10.10

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.