Antiviral effect of palmatine against infectious bronchitis virus through regulation of NF-κB/IRF7/JAK-STAT signalling pathway and apoptosis.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

British Poultry Science Pub Date : 2024-04-01 Epub Date: 2024-01-26 DOI:10.1080/00071668.2023.2296929

W Zhang, Y Chen, F Yang, H Zhang, T Su, J Wang, Y Zhang, X Song

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引用次数: 0

Abstract

1. Infectious bronchitis virus (IBV), a gamma-coronavirus, can infect chickens of all ages and leads to an acute contact respiratory infection. This study evaluated the anti-viral activity of palmatine, a natural non-flavonoid alkaloid, against IBV in chicken embryo kidney (CEK) cells.2. The half toxic concentration (CC₅₀) of palmatine was 672.92 μM, the half inhibitory concentration (IC₅₀) of palmatine against IBV was 7.76 μM and the selection index (SI) was 86.74.3. Mode of action assay showed that palmatine was able to directly inactivate IBV and inhibited the adsorption, penetration and intracellular replication of IBV.4. Palmatine significantly upregulated TRAF6, TAB1 and IKK-β compared with the IBV-infected group, leading to the increased expressions of pro-inflammatory cytokines IL-1β and TNF-α in the downstream NF-κB signalling pathway.5. Palmatine significantly up-regulated the levels of MDA5, MAVS, IRF7, IFN-α and IFN-β in the IRF7 pathway, inducing type I interferon production. It up-regulated the expression of 2'5'-oligoadenylate synthase (OAS) in the JAK-STAT pathway.6. IBV infection induced cell apoptosis and palmatine-treatment delayed the process of apoptosis by regulation of the expression of apoptosis-related genes (BAX, BCL-2, CASPASE-3 and CASPASE-8).7. Palmatine could exert anti-IBV activity through regulation of NF-κB/IRF7/JAK-STAT signalling pathways and apoptosis, providing a theoretical basis for the utilisation of palmatine to treat IBV infection.

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巴马汀通过调节 NF-κB/IRF7/JAK-STAT 信号通路和细胞凋亡对传染性支气管炎病毒的抗病毒作用

1.传染性支气管炎病毒（IBV）是一种γ-冠状病毒，可感染所有年龄的鸡，并导致急性接触性呼吸道感染。2. 巴马汀的半毒浓度（CC50）为 672.92 μM，对 IBV 的半抑制浓度（IC50）为 7.76 μM，选择指数（SI）为 86.74.3。作用模式分析显示，巴马汀能够直接灭活 IBV，并抑制 IBV 的吸附、穿透和细胞内复制。与 IBV 感染组相比，巴马汀能明显上调 TRAF6、TAB1 和 IKK-β，导致下游 NF-κB 信号通路中促炎细胞因子 IL-1β 和 TNF-α 的表达增加。巴马汀能明显上调 IRF7 通路中 MDA5、MAVS、IRF7、IFN-α 和 IFN-β的水平，诱导 I 型干扰素的产生。它上调了 JAK-STAT 通路中 2'5'-oligoadenylate synthase（OAS）的表达。巴马汀可通过调节 NF-κB/IRF7/JAK-STAT 信号通路和细胞凋亡发挥抗 IBV 活性，为利用巴马汀治疗 IBV 感染提供了理论依据。

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来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .