Prevalence of Indigenous Antibiotic-Resistant Salmonella Isolates and Their Application to Explore a Lytic Phage vB_SalS_KFSSM with an Intra-Broad Specificity

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-01-02 DOI:10.1007/s12275-023-00098-6

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Abstract

The consumption of fresh produce has led to increase in antibiotic-resistant (AR) Salmonella outbreaks. In this study, indigenous Salmonella was isolated from a total of two hundred-two samples including fresh produce and agricultural environmental samples in Korea. After biochemical confirmation using the Indole, Methyl Red, Voges-Proskauer, Citrate tests, presumable Salmonella isolates were identified by 16S rRNA sequencing. Identified Salmonella isolates were evaluated for antibiotic susceptibility against twenty-two antibiotics. The specificity and the efficiency of plating (EOP) of vB_SalS_KFSSM were evaluated against fifty-three bacterial strains. Twenty-five suspected Salmonella were isolated and confirmed by the positive result for methyl red and citrate, of which ten were identified as Salmonella spp. through 16S rRNA gene sequencing. Eight Salmonella isolates (4.0%, n = 8/202) were resistant to at least one antibiotic, among which five were multi-drug resistant. As a lytic phage against Salmonella spp. CMGS-1, vB_SalS_KFSSM was isolated from cow manure. The phage was observed as a tailed phage belonging to the class Caudoviricetes. It exhibited an intra-broad specificity against four indigenous AR Salmonella isolates, two indigenous Salmonella isolates, and five other Salmonella serotypes with great efficiencies (EOP ≥ 0.75). Thus, this study suggested the potential of vB_SalS_KFSSM to combat indigenous AR Salmonella.

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本地抗生素耐药沙门氏菌分离物的流行及其在探索具有广谱特异性的溶菌噬菌体 vB_SalS_KFSSM 中的应用

摘要食用新鲜农产品导致抗生素耐药性沙门氏菌爆发增加。本研究从韩国新鲜农产品和农业环境样本共 222 份样本中分离出本土沙门氏菌。在使用吲哚、甲基红、Voges-Proskauer 和柠檬酸盐测试进行生化确认后，通过 16S rRNA 测序鉴定了可推定的沙门氏菌分离物。对鉴定出的沙门氏菌分离物进行了抗生素敏感性评估，共检测了 22 种抗生素。评估了 vB_SalS_KFSSM 对 53 种细菌菌株的特异性和培养效率（EOP）。分离出 25 株疑似沙门氏菌，并通过甲基红和柠檬酸盐的阳性结果进行了确认，其中 10 株通过 16S rRNA 基因测序被确定为沙门氏菌属。分离出的 8 个沙门氏菌（4.0%，n = 8/202）对至少一种抗生素具有耐药性，其中 5 个具有多重耐药性。从牛粪中分离出针对沙门氏菌属 CMGS-1 的溶菌噬菌体 vB_SalS_KFSSM。据观察，该噬菌体属于尾状噬菌体（Caudoviricetes）。它对 4 个本地 AR 沙门氏菌分离物、2 个本地沙门氏菌分离物和 5 个其他沙门氏菌血清型具有广泛的特异性，且效率极高（EOP ≥ 0.75）。因此，这项研究表明 vB_SalS_KFSSM 具有抗本地 AR 沙门氏菌的潜力。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567