Mapping QTLs for grain iron, zinc, and yield traits in advanced backcross inbred lines of Samba mahsuri (BPT5204)/Oryza rufipogon

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-01-02 DOI:10.1007/s13562-023-00869-7

Gowthami Chandu, Divya Balakrishnan, Suchandranath Babu Munnam, Satendra K. Mangrauthia, Durbha Sanjeeva Rao, C. N. Neeraja, R. M. Sundaram, Sarla Neelamraju

{"title":"Mapping QTLs for grain iron, zinc, and yield traits in advanced backcross inbred lines of Samba mahsuri (BPT5204)/Oryza rufipogon","authors":"Gowthami Chandu, Divya Balakrishnan, Suchandranath Babu Munnam, Satendra K. Mangrauthia, Durbha Sanjeeva Rao, C. N. Neeraja, R. M. Sundaram, Sarla Neelamraju","doi":"10.1007/s13562-023-00869-7","DOIUrl":null,"url":null,"abstract":"Iron and zinc deficiency is a major problem among large populations in rice-consuming countries. Development micronutrient dense rice varieties with high yield is a key target area in breeding programmes and QTL mapping studies using backcross inbred lines to transfer beneficial genes from wild relatives is one of the potential strategy. In this study, 136 BC4F10 backcross inbred lines (BILs) from BPT5204 x Oryza rufipogon WR119 were field evaluated for 3 years for nine yield related traits. Grain Fe and Zn were estimated using ED-XRF. In all, 11 major QTLs with phenotypic variance from 10 to 16.8% were identified for Fe, Zn, and 5 yield related traits. O. rufipogon alleles were trait-enhancing in 18% of all QTLs and an allele at qFe2.1 increased iron concentration. Major effect QTLs qFe1.1 for grain Fe and qZn5.1, qZn8.1, and qZn10.1 for grain Zn explained 11 to 16% PVE, qZn8.1 and qZn10.1 were co-located with QTLs for grain yield related traits. Seven chromosomal regions showed QTLs for more than two traits. QTLs were associated with several high priority candidate genes for grain Fe, Zn and yield. One elite BIL [IET 24775 RP4920-Bio51B] was tested in AICRIP bio fortification trials for 4 years [2014–2017], and three BILs [IET 28715 RP4920-Bio61-1B], [IET28706 RP4920-Bio83B] and [IET28695 RP4920-Bio88B] are evaluated for 2 years of trials. The significant BILs and QTLs are useful in rice bio fortification and for gene discovery.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s13562-023-00869-7","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Iron and zinc deficiency is a major problem among large populations in rice-consuming countries. Development micronutrient dense rice varieties with high yield is a key target area in breeding programmes and QTL mapping studies using backcross inbred lines to transfer beneficial genes from wild relatives is one of the potential strategy. In this study, 136 BC₄F₁₀ backcross inbred lines (BILs) from BPT5204 x Oryza rufipogon WR119 were field evaluated for 3 years for nine yield related traits. Grain Fe and Zn were estimated using ED-XRF. In all, 11 major QTLs with phenotypic variance from 10 to 16.8% were identified for Fe, Zn, and 5 yield related traits. O. rufipogon alleles were trait-enhancing in 18% of all QTLs and an allele at qFe2.1 increased iron concentration. Major effect QTLs qFe1.1 for grain Fe and qZn5.1, qZn8.1, and qZn10.1 for grain Zn explained 11 to 16% PVE, qZn8.1 and qZn10.1 were co-located with QTLs for grain yield related traits. Seven chromosomal regions showed QTLs for more than two traits. QTLs were associated with several high priority candidate genes for grain Fe, Zn and yield. One elite BIL [IET 24775 RP4920-Bio51B] was tested in AICRIP bio fortification trials for 4 years [2014–2017], and three BILs [IET 28715 RP4920-Bio61-1B], [IET28706 RP4920-Bio83B] and [IET28695 RP4920-Bio88B] are evaluated for 2 years of trials. The significant BILs and QTLs are useful in rice bio fortification and for gene discovery.

Abstract Image

查看原文本刊更多论文

绘制高级回交近交系 Samba mahsuri (BPT5204)/Oryza rufipogon 谷物铁、锌和产量性状的 QTLs 图谱

缺铁和缺锌是水稻消费国大量人口面临的主要问题。育种计划的一个关键目标领域是开发微量营养素含量高且产量高的水稻品种，而利用回交近交系从野生近缘植物中转移有益基因的 QTL 图谱研究则是潜在的策略之一。本研究对 136 个 BC4F10 回交近交系（BILs）进行了为期 3 年的田间评估，这些近交系来自 BPT5204 x Oryza rufipogon WR119，涉及 9 个与产量相关的性状。谷物中铁和锌的含量是用 ED-XRF 估算的。在铁、锌和 5 个产量相关性状方面，共鉴定出 11 个主要 QTL，其表型方差在 10% 到 16.8% 之间。在所有 QTL 中，有 18% 的 O. rufipogon 等位基因对性状有增强作用，qFe2.1 等位基因可提高铁的浓度。谷粒铁的主要效应 QTL qFe1.1，谷粒锌的 qZn5.1、qZn8.1 和 qZn10.1 解释了 11% 至 16% 的 PVE，qZn8.1 和 qZn10.1 与谷粒产量相关性状的 QTL 位于同一位置。有 7 个染色体区域出现了两个以上性状的 QTLs。QTL 与谷物铁、锌和产量的几个优先候选基因相关。一个精英 BIL [IET 24775 RP4920-Bio51B]在 AICRIP 生物强化试验中进行了 4 年试验 [2014-2017]，三个 BIL [IET 28715 RP4920-Bio61-1B]、[IET28706 RP4920-Bio83B]和[IET28695 RP4920-Bio88B]进行了 2 年试验评估。这些重要的 BIL 和 QTLs 对水稻生物强化和基因发掘非常有用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.