Moraea sisyrinchium inhibits proliferation, cell cycle, and migration of cancerous cells, and decreases angiogenesis in chick chorioallantoic membrane.

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Roghayeh Rashidi, Ala Montazeri, Mohammad Soukhtanloo, Shirin Ghasemian, Mohammad Sadegh Amiri, Maede Hasanpour, Elham Einafshar, Ahmad Ghorbani
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引用次数: 0

Abstract

Objectives: Experimental studies reported that some plants in the genus of Moraea (Iridaceae family) show anticancer potential. This study aimed to evaluate the effects of Moraea sisyrinchium on U87 glioblastoma multiforme and HepG2 liver cancer cells.

Materials and methods: The cells were incubated for 24 hr with hydroalcoholic extract of the stem, flower, and bulb of M. sisyrinchium. Then, the cell proliferation (MTT) assay, cell cycle analysis (propidium iodide staining), cell migration test (scratch), Western blotting (Bax and Bcl-2 expression), and gelatin zymography (for matrix metalloproteinases, MMPs) were performed. Oxidative stress was evaluated by determining the levels of reactive oxygen species and lipid peroxidation. Angiogenesis was evaluated on chick embryo chorioallantoic membrane.

Results: The extracts of the flower, stem, and bulb significantly decreased the proliferation of HepG2 and U87 cells. This effect was more for U87 than HepG2 and for the bulb and stem than the flower. In U87 cells, the bulb extract increased oxidative stress, cell cycle arrest, and the Bax/Bcl-2 ratio. Also, this extract suppressed the migration ability of HepG2 and U87 cells, which was associated with the inhibition of MMP2 activity. In addition, it significantly reduced the number and diameter of vessels in the chorioallantoic membrane. Liquid chromatography-mass spectrometry revealed the presence of xanthones (bellidifolin and mangiferin), flavonoids (quercetin and luteolin), isoflavones (iridin and tectorigenin), and phytosterols (e.g., stigmasterol) in the bulb.

Conclusion: M. sisyrinchium bulb decreased the proliferation and survival of cancer cells by inducing oxidative stress. It also reduced the migration ability of the cells and inhibited angiogenesis.

Moraea sisyrinchium 能抑制癌细胞的增殖、细胞周期和迁移,并能减少小鸡绒毛膜血管生成。
目的:实验研究表明,桑属(鸢尾科)的一些植物具有抗癌潜力。本研究旨在评估 Moraea sisyrinchium 对 U87 多形性胶质母细胞瘤和 HepG2 肝癌细胞的影响:将细胞与莫来菌素的茎、花和鳞茎的水醇提取物培养 24 小时。然后,进行细胞增殖(MTT)试验、细胞周期分析(碘化丙啶染色)、细胞迁移试验(划痕)、Western 印迹(Bax 和 Bcl-2 表达)和明胶酶谱分析(基质金属蛋白酶)。通过测定活性氧和脂质过氧化物的水平来评估氧化应激。在小鸡胚胎绒毛膜上对血管生成进行了评估:结果:花、茎和球茎的提取物能明显减少 HepG2 和 U87 细胞的增殖。对 U87 的影响大于对 HepG2 的影响,对球茎和茎的影响大于对花的影响。在 U87 细胞中,球茎提取物增加了氧化应激、细胞周期停滞和 Bax/Bcl-2 比率。鳞茎提取物还抑制了 HepG2 和 U87 细胞的迁移能力,这与抑制 MMP2 活性有关。此外,它还能明显减少绒毛膜中血管的数量和直径。液相色谱-质谱分析表明,球茎中含有黄酮类化合物(贝利得福林和芒果苷)、黄酮类化合物(槲皮素和木犀草素)、异黄酮类化合物(鸢尾黄素和矢车菊黄素)和植物甾醇(如豆甾醇):结论:水飞蓟鳞茎通过诱导氧化应激减少了癌细胞的增殖和存活。结论:水飞蓟鳞茎通过诱导氧化应激降低了癌细胞的增殖和存活率,还降低了细胞的迁移能力并抑制了血管生成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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