Characterization of gut microbiome profile in children with confirmed wheat allergy.

IF 2.3 4区 医学 Q3 ALLERGY
Watcharoot Kanchongkittiphon, Saifon Nopnipa, Rubwad Mathuranyanon, Nutthawan Nonthabenjawan, Suphanich Sritournok, Wiparat Manuyakorn, Pagakrong Wanapaisan
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引用次数: 0

Abstract

Background: Food allergies pose serious health risks, including life-threatening anaphylactic reactions, increased morbidity, and reduced quality of life. Wheat allergy is a common concern in Asia. There is growing interest in understanding the potential association between dysregulation of the gut microbiome and the development of food allergies.

Objective: This study aimed to explore the gut microbiome of Thai children with wheat allergy and its potential association with allergic responses.

Methods: Microbial abundance was assessed using Quantitative Insights into Microbial Ecology 2 (QIIME2) microbiome analysis based on 16S rDNA data. The correlation between microbial richness and relevant parameters was evaluated using the Spearman correlation analysis. Additionally, the microbial community functions were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2).

Results: The gut microbiome analysis revealed significant differences between the two groups at the phylum and genus levels. Firmicutes (p = 0.012) and Verrucomicrobia (p < 0.001) were enriched in wheat-allergic children, whereas specific gut microbes such as Megamonas (p = 0.04), Romboutsia (p < 0.001), Fusobacterium (p < 0.001), Clostridium senso stricto1 (p < 0.001), and Turicibacter (p < 0.001) were more abundant in healthy children. Anaerostripes (p = 0.011), Erysipelatoclostridium (p < 0.001), Prevotella 2 (p < 0.001), Ruminiclostridium 5 (p < 0.001), and Clostridium innnocuum (p < 0.001) were enriched in children with a confirmed wheat allergy. Functional analysis indicated disparities in the pathways related to arginine and polyamine biosynthesis.

Conclusion: These findings offer valuable insights into the gut microbiome of children with wheat allergy and its potential impact on symptom severity, laying the groundwork for further research and interventions aimed at addressing this health concern.

确诊对小麦过敏的儿童肠道微生物组特征。
背景:食物过敏会对健康造成严重危害,包括危及生命的过敏反应、发病率增加和生活质量下降。小麦过敏是亚洲人普遍关注的问题。人们越来越有兴趣了解肠道微生物组失调与食物过敏发生之间的潜在联系:本研究旨在探讨泰国小麦过敏儿童的肠道微生物组及其与过敏反应的潜在关联:方法:使用基于 16S rDNA 数据的微生物组定量分析 2 (QIIME2) 评估微生物丰度。利用斯皮尔曼相关分析评估了微生物丰富度与相关参数之间的相关性。此外,还利用未观察状态重建的群落系统发育调查(PICRUSt2)对微生物群落功能进行了预测:结果:肠道微生物组分析表明,两组在门和属的水平上存在显著差异。小麦过敏症儿童体内富含固着菌(p = 0.012)和疣菌(p < 0.001),而特定的肠道微生物,如Megamonas(p = 0.04)、Romboutsia(p < 0.001)、Fusobacterium(p < 0.001)、Clostridium senso stricto1(p < 0.001)和Turisibacter(p < 0.001)在健康儿童体内更为丰富。厌氧菌(p = 0.011)、红细胞梭菌(p < 0.001)、普雷沃特氏菌 2(p < 0.001)、反刍梭菌 5(p < 0.001)和innocuum梭菌(p < 0.001)在确诊小麦过敏的儿童中富集。功能分析显示,与精氨酸和多胺生物合成相关的通路存在差异:这些发现为了解小麦过敏儿童的肠道微生物组及其对症状严重程度的潜在影响提供了宝贵的信息,为进一步研究和干预这一健康问题奠定了基础。
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来源期刊
CiteScore
12.80
自引率
0.00%
发文量
74
审稿时长
>12 weeks
期刊介绍: The Asian Pacific Journal of Allergy and Immunology (APJAI) is an online open access journal with the recent impact factor (2018) 1.747 APJAI published 4 times per annum (March, June, September, December). Four issues constitute one volume. APJAI publishes original research articles of basic science, clinical science and reviews on various aspects of allergy and immunology. This journal is an official journal of and published by the Allergy, Asthma and Immunology Association, Thailand. The scopes include mechanism, pathogenesis, host-pathogen interaction, host-environment interaction, allergic diseases, immune-mediated diseases, epidemiology, diagnosis, treatment and prevention, immunotherapy, and vaccine. All papers are published in English and are refereed to international standards.
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