Synseed germination, conversion, and acclimatization of seedless watermelon ‘Quality’

IF 2.2 3区 生物学 Q4 CELL BIOLOGY
Putri Santika, Jong-Yi Fang
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Abstract

Seedless watermelons are triploid hybrids (3n) that have been growing in popularity since the 1990s. However, the high production cost and low germination rate of the hybrid seeds pose a major problem in their production. The synseed technology could make a promising alternative for seedless watermelon propagation; however, this alternative has not yet been explored. This study aimed at producing synseeds of seedless watermelon ‘Quality’ using shoot tips as the propagules after which their germination and conversion were evaluated under in vitro and ex vitro conditions. In vitro shoot multiplication with different concentrations of 6-benzylaminopurine (BAP), synseed production from BAP-multiplied shoot tips, synseed sowing in vitro using several substrates and medium supplements, and synseed sowing ex vitro using different antimicrobial agents were investigated. Results indicated that 1.0 mg L−1 BAP resulted in the highest number of shoots per shoot tip explant. Synseeds produced from BAP-multiplied shoot tips achieved faster germination compared to synseeds from non-BAP-derived shoot tips, but there was no difference in terms of conversion and acclimatization survival rates. Agar showed greater conversion and acclimatization survival rates as well as root number, root length, and shoot length than the other substrates especially when indole-3-butyric acid (IBA) at 1.0 mg L−1 was added to the medium. However, this study demonstrated the possibility of using vermiculite as an alternative substrate to agar. PPM at 0.5% was a better microbial agent than 100.0 mg L−1 mancozeb for ex vitro synseed sowing as it achieved zero contamination and maintained the growing potential of synseeds with 65% germination, 85% survival, and 25% rooting rates.

Abstract Image

无籽西瓜 "优质 "的同种发芽、转化和适应性培养
无籽西瓜是三倍体杂交种(3n),自 20 世纪 90 年代以来越来越受欢迎。然而,杂交种子的生产成本高、发芽率低,是其生产中的一个主要问题。同系种子技术可以作为无籽西瓜繁殖的一种很有前途的替代技术;然而,这种替代技术尚未得到探索。这项研究的目的是利用芽尖作为繁殖体生产无籽西瓜 "优质 "的合子,然后在离体和体外条件下评估其发芽和转化情况。研究了使用不同浓度的 6-苄基氨基嘌呤(BAP)进行的离体芽繁殖、BAP 繁殖的芽尖产生的合子、使用几种基质和培养基补充剂进行的合子离体播种以及使用不同抗菌剂进行的合子离体播种。结果表明,1.0 mg L-1 BAP 使每个芽尖外植体产生的芽数量最多。与不使用 BAP 的嫩梢相比,使用 BAP 繁殖的嫩梢产生的同种异花种子发芽更快,但在转化率和适应存活率方面没有差异。琼脂的转化率和适应存活率以及根数、根长和芽长均高于其他基质,尤其是在培养基中添加 1.0 mg L-1 的吲哚-3-丁酸(IBA)时。不过,这项研究证明了使用蛭石作为琼脂替代基质的可能性。与 100.0 mg L-1 代森锰锌相比,0.5% 的 PPM 是一种更好的微生物制剂,因为它可以实现零污染,并保持代森锰锌的生长潜力,发芽率为 65%,存活率为 85%,生根率为 25%。
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来源期刊
CiteScore
5.00
自引率
7.70%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.
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