A virus from Aspergillus cibarius with features of alpha- and betachrysoviruses

IF 1.9 4区医学 Q3 GENETICS & HEREDITY

Virus Genes Pub Date : 2023-12-30 DOI:10.1007/s11262-023-02043-6

Tobias Lutz, Gitta Jutta Langer, Cornelia Heinze

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Abstract

From the ascomycete Aspergillus cibarius strain NW-FVA 2590, which was originally isolated from a root, associated with stem collar necrosis of Fraxinus excelsior L., a novel virus was isolated and characterized. Its genome is encoded on three monocistronic dsRNA segments ranging from 3683 bp (dsRNA 1) over 3093 (dsRNA 2) to 2902 bp (dsRNA 3), which are packed in isometric particles of around 35 nm. While the viral RdRp (P1) is encoded on segment 1, protein sequencing showed that two more structural proteins are present which are translated from dsRNA 2 (P2) and dsRNA 3 (P3) and possibly form the viral capsid. Additionally, P2 and P3 may undergo posttranslational modifications since the detected proteins bands deviated from the calculated sizes. Due to its phylogenetic position, the novel virus was grouped in the family of Chrysoviridae and was tentatively denominated as Aspergillus cibarius chrysovirus 1 (AcCV1). Due to its composition, biological properties and phylogenetic position, distant from the genera Alphachrysovirus and Betachrysovirus, we suggest to position AcCV1 in a proposed genus “Gammachrysovirus”.

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一种来自西巴里曲霉的病毒，具有甲型和乙型鲎病毒的特征

从最初从根部分离出的与梣树（Fraxinus excelsior L.）茎领坏死有关的曲霉菌株 NW-FVA 2590 中，分离出了一种新型病毒并对其进行了鉴定。它的基因组由三个单核苷酸 dsRNA 片段编码，范围从 3683 bp（dsRNA 1）到 3093 bp（dsRNA 2）再到 2902 bp（dsRNA 3），这些片段被包装在 35 nm 左右的等距颗粒中。病毒 RdRp（P1）编码在 1 号片段上，蛋白质测序结果显示，还有两种结构蛋白存在，它们分别由 dsRNA 2（P2）和 dsRNA 3（P3）翻译而来，并可能形成病毒壳。此外，P2 和 P3 可能经过翻译后修饰，因为检测到的蛋白质条带与计算的大小有偏差。由于其系统发育的位置，该新型病毒被归入克里索病毒科，并被暂定为西巴里曲霉克里索病毒 1（Acpergillus cibarius chrysovirus 1，AcCV1）。由于其组成、生物学特性和系统发育位置与 Alphachrysovirus 属和 Betachrysovirus 属相去甚远，我们建议将 AcCV1 归入拟议的 "Gammachrysovirus "属。

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来源期刊

Virus Genes 医学-病毒学

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Viruses are convenient models for the elucidation of life processes. The study of viruses is again on the cutting edge of biological sciences: systems biology, genomics, proteomics, metagenomics, using the newest most powerful tools. Huge amounts of new details on virus interactions with the cell, other pathogens and the hosts – animal (including human), insect, fungal, plant, bacterial, and archaeal - and their role in infection and disease are forthcoming in perplexing details requiring analysis and comments. Virus Genes is dedicated to the publication of studies on the structure and function of viruses and their genes, the molecular and systems interactions with the host and all applications derived thereof, providing a forum for the analysis of data and discussion of its implications, and the development of new hypotheses.