Insulin-like growth factor-1 expression levels in pro-inflammatory response in calves with neonatal systemic inflammatory response syndrome

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2023-12-27 DOI:10.1016/j.vetimm.2023.110706

Kerim Emre Yanar , Cihan Gür , Şükrü Değirmençay , Ömer Aydın , Mustafa Sinan Aktaş , Sümeyye Baysal

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引用次数: 0

Abstract

The objective of this study was to investigate the mRNA expression of insulin-like growth factor-1 (IGF-1), pro-inflammatory cytokines (IL-1β, IL-6, IL-18, and TNF-α), serum immunoglobulin profiles (IgG and IgM), and lipid peroxidation status (MDA) in relation to pro-inflammatory cytokines. A case-controlled, prospective, and observational investigation was completed on 85 calves. Total RNA was isolated from whole blood samples of both the SIRS and healthy calves, followed by reverse transcription into cDNA. The resulting cDNAs were mixed with iTaq Universal SYBR Green Supermix and primers specific to the relevant genes using the Rotor-Gene Q instrument. After the reaction was completed, gene expressions were normalised against β-actin using the 2^-ΔΔCT method. The mRNA levels of pro-inflammatory cytokines namely (IL-1β [SIRS: 2.15 ± 0.55, Control: 1.13 ± 0.62; P = 0.001], IL-6 [SIRS: 2.82 ± 0.52, Control: 0.91 ± 0.11; P < 0.001], IL-18 [SIRS: 1.92 ± 0.41, Control: 0.99 ± 0.13; P < 0.001], and TNF-α [SIRS: 2.59 ± 0.28, Control: 0.93 ± 0.09; P < 0.001]) and IGF-1 (SIRS: 3.55 ± 0.55, Control: 0.91 ± 0.15; P < 0.001) were up-regulated in calves with SIRS, while serum IgG (SIRS: 4.16 ± 0.26, Control: 1.73 ± 0.17; P < 0.001), IgM (SIRS: 1.55 ± 0.11, Control: 1.09 ± 0.13; P < 0.001), and MDA levels (SIRS: 41.12 ± 3.48, Control: 3.76 ± 0.81; P < 0.001) increased significantly in these calves. Furthermore, significant (P < 0.01) positive correlations were found in calves with SIRS in relation to the expression levels of IL-1β, IL-6, IL-18, TNF-α, IGF-1, serum immunoglobulins, and MDA levels. These results suggest that IGF-1 could be a valuable pro-inflammatory marker, considering its high positive correlation with the expression levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-18, and TNF-α) and markers (MDA, IgG, and IgM) in calves with SIRS.

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新生儿全身炎症反应综合征犊牛促炎症反应中胰岛素样生长因子-1的表达水平

本研究的目的是调查胰岛素样生长因子-1（IGF-1）、促炎细胞因子（IL-1β、IL-6、IL-18 和 TNF-α）、血清免疫球蛋白（IgG 和 IgM）的 mRNA 表达以及脂质过氧化状态（MDA）与促炎细胞因子的关系。对 85 头小牛进行了病例对照、前瞻性和观察性调查。从 SIRS 小牛和健康小牛的全血样本中分离出总 RNA，然后反转录成 cDNA。使用 Rotor-Gene Q 仪器将得到的 cDNA 与 iTaq Universal SYBR Green Supermix 和相关基因的特异引物混合。反应结束后，使用 2-ΔΔCT 方法将基因表达量与β-肌动蛋白进行归一化。促炎细胞因子的 mRNA 水平分别为：IL-1β [SIRS: 2.15 ± 0.55, Control: 1.13 ± 0.62; P = 0.001]、IL-6 [SIRS: 2.82 ± 0.52, Control: 0.91 ± 0.11; P < 0.001]、IL-18 [SIRS: 1.92 ± 0.41, 对照组: 0.99 ± 0.13; P < 0.001]、TNF-α [SIRS: 2.59 ± 0.28, 对照组: 0.93 ± 0.09; P < 0.001]）和 IGF-1 （SIRS: 3.55 ± 0.55，对照组：0.91 ± 0.15；P <；0.001]）上调，而血清 IgG（SIRS：4.16 ± 0.26，对照组：1.73 ± 0.17；P <；0.001]）、IgM（SIRS：1.55 ± 0.11，对照组：1.09 ± 0.13；P <；0.001）和 MDA 水平（SIRS：41.12 ± 3.48，对照组：3.76 ± 0.81；P <；0.001）显著增加。此外，在患有 SIRS 的犊牛中发现，IL-1β、IL-6、IL-18、TNF-α、IGF-1、血清免疫球蛋白和 MDA 水平的表达水平呈明显的正相关（P < 0.01）。这些结果表明，考虑到IGF-1与SIRS犊牛的促炎细胞因子（IL-1β、IL-6、IL-18和TNF-α）和标记物（MDA、IgG和IgM）的表达水平高度正相关，IGF-1可能是一种有价值的促炎标记物。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.