The role of intracellular β-glucosidase in cellulolytic response induction in filamentous fungus Penicillium verruculosum

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2024-05-01 DOI:10.1016/j.resmic.2023.104178

Valeriy Yu Kislitsin , Andrey M. Chulkin , Anna S. Dotsenko , Igor G. Sinelnikov , Arkady P. Sinitsyn , Aleksandra M. Rozhkova

引用次数: 0

Abstract

In this study, CRISPR/Cas9 genome editing was used to knockout the bgl2 gene encoding intracellular β-glucosidase filamentous fungus Penicillium verruculosum. This resulted in a dramatic reduction of secretion of cellulolytic enzymes. The study of P. verruculosum Δbgl2 found that the transcription of the cbh1 gene, which encodes cellobiohydrolase 1, was impaired when induced by cellobiose and cellotriose. However, the transcription of the cbh1 gene remains at level of the host strain when induced by gentiobiose. This implies that gentiobiose is the true inducer of the cellulolytic response in P. verruculosum, in contrast to Neurospora crassa where cellobiose acts as an inducer.

查看原文本刊更多论文

丝状真菌疣青霉胞内β-葡萄糖苷酶在纤维素分解反应诱导中的作用

在这项研究中，利用 CRISPR/Cas9 基因组编辑技术敲除了编码胞内β-葡萄糖苷酶丝状真菌疣青霉的 bgl2 基因。这导致纤维素分解酶的分泌量急剧减少。对 P. verruculosum Δbgl2 的研究发现，在纤维二糖和纤维三糖的诱导下，编码纤维二糖水解酶 1 的 cbh1 基因的转录受到影响。然而，在龙胆双糖的诱导下，cbh1 基因的转录仍保持宿主菌株的水平。这意味着龙胆三糖才是疣梭菌纤维素分解反应的真正诱导剂，而在克氏黑孢子（Neurospora crassa）中，纤维三糖才是诱导剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.