MicroRNA profiling in complete and partial hydatidiform moles.

IF 0.6 4区医学 Q4 PATHOLOGY

Malaysian Journal of Pathology Pub Date : 2023-12-01

W K Chia, H X Y Yeoh, M Mustangin, T Y Khong, Y P Wong, G C Tan

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引用次数: 0

Abstract

Introduction: Hydatidiform mole is one of the gestational trophoblastic disease and comprises complete (CM) and partial moles (PM), which carries a risk of developing persistence disease, invasive mole or choriocarcinoma. MicroRNAs (miRNAs) have been discovered in various tissues, including neoplastic tissues. Its role in the pathogenesis of molar pregnancy or as biomarkers are still largely uncertain. The aim of this study is to identify the differentially expressed miRNAs in CM and PM.

Materials and methods: Using next-generation sequencing, the miRNAs profiles of CM (n=3) and PM (n=3) moles, including placenta of non-molar abortus (n=3) as control were determined. The differentially expressed miRNAs between each group were analysed. Subsequently, bioinformatics analysis using miRDB and Targetscan was utilised to predict target genes.

Results: We found 10 differentially expressed miRNAs in CMs and PMs, compared to NMAs, namely miR- 518a-5p, miR-423-3p, miR-503-5p, miR-302a-3p, and miR-1323. The other 5 miRNAs were novel, not listed in the known database. The 3 differentially expressed miRNAs in CMs were predicted to commonly target ZTBT46 and FAM73B mRNAs.

Discussion: miR-518 was consistently observed to be downregulated in CM versus PM, and CM versus NMA. Further bioinformatic analysis to provide insight into the possible role of these miRNAs in the pathogenesis of HMs, progression of disease and as potential diagnostic biomarkers as well as therapeutic targets for HMs is needed.

本刊更多论文

完全和部分水滴形痣的微RNA图谱分析。

导言：水滴形痣是妊娠滋养细胞疾病之一，包括完全痣（CM）和部分痣（PM），有发展为顽固性疾病、浸润性痣或绒毛膜癌的风险。在包括肿瘤组织在内的各种组织中都发现了微小核糖核酸（miRNAs）。其在恶性妊娠发病机制中的作用或作为生物标志物的作用在很大程度上仍不确定。本研究旨在确定 CM 和 PM 中表达不同的 miRNA：采用新一代测序技术，测定了CM（n=3）和PM（n=3）痣的miRNAs谱，包括作为对照的非大疱性流产胎盘（n=3）。分析了各组之间差异表达的 miRNA。随后，利用 miRDB 和 Targetscan 进行生物信息学分析，预测靶基因：结果：与 NMAs 相比，我们在 CMs 和 PMs 中发现了 10 个不同表达的 miRNA，即 miR-518a-5p、miR-423-3p、miR-503-5p、miR-302a-3p 和 miR-1323。另外 5 个 miRNA 是新的 miRNA，未列入已知数据库。在 CM 中差异表达的 3 个 miRNA 被预测为通常以 ZTBT46 和 FAM73B mRNA 为靶标。需要进一步进行生物信息学分析，以深入了解这些 miRNA 在 HMs 发病机制、疾病进展以及作为 HMs 潜在诊断生物标志物和治疗靶点中可能发挥的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Malaysian Journal of Pathology PATHOLOGY-

CiteScore

3.60

自引率

5.60%

发文量

期刊介绍： The Malaysian Journal of Pathology is the official journal of the College of Pathologists, Academy of Medicine Malaysia. The primary purpose of The Journal is to publish the results of study and research in Pathology, especially those that have particular relevance to human disease occurring in Malaysia and other countries in this region. The term PATHOLOGY will be interpreted in its broadest sense to include Chemical Pathology, Cytology, Experimental Pathology, Forensic Pathology, Haematology, Histopathology, Immunology, Medical Microbiology and Parasitology. The Journal aims to bring under one cover publications of regional interest embracing the various sub-specialities of Pathology. It is expected that the articles published would be of value not only to pathologists, but also to medical practitioners in search of a scientific basis for the problems encountered in their practice, and to those with an interest in diseases which occur in the tropics.