Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans.

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2023-12-01 DOI:10.18502/ijm.v15i6.14162

Amira Habibi, Mansoura Bayat, Behinb Omidi, Ali Ezabadi, Pejmana Mortazavi

{"title":"Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans.","authors":"Amira Habibi, Mansoura Bayat, Behinb Omidi, Ali Ezabadi, Pejmana Mortazavi","doi":"10.18502/ijm.v15i6.14162","DOIUrl":null,"url":null,"abstract":"Background and objectives: Candida albicans cause a problematic condition in immunocompromised patients that could not be treated quickly due to the resistant behavior of microorganisms. This study aimed to investigate the effect of a novel ionic liquid (IL) as a new drug on C. albicans strains.Materials and methods: Seven newly binary ionic liquids mixtures were synthesized, and among them, ([prollinium chloride] [1-methylimidazolium 3-sulfonate] ([pro-HCl][MImS]) was selected and characterized by 1HNMR, 13C NMR, and FT-IR methods. Samples from patients (n=50) with candidiasis were collected and identified through culture media. ERG11 gene overexpression was related to resistance against azole-bearing drugs. The antibiogram, well diffusion assay, MICs, and MFCs tests were operated. PCR and Real-time evaluated the expression of the ERG11 gene, and the rate of cell death was detected using Flow Cytometry.Results: Our data manifested that this novel IL (Ionic Liquid) can inhibit C. albican's growth, reduce the expression of ERG11 and increase dead cells.Conclusion: The newly synthesized IL had an inhibiting effect on the growth of the C. albicans strains and may be used as an alternative candidate for novel drug design.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 6","pages":"811-820"},"PeriodicalIF":1.3000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10751616/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v15i6.14162","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background and objectives: Candida albicans cause a problematic condition in immunocompromised patients that could not be treated quickly due to the resistant behavior of microorganisms. This study aimed to investigate the effect of a novel ionic liquid (IL) as a new drug on C. albicans strains.

Materials and methods: Seven newly binary ionic liquids mixtures were synthesized, and among them, ([prollinium chloride] [1-methylimidazolium 3-sulfonate] ([pro-HCl][MImS]) was selected and characterized by 1HNMR, 13C NMR, and FT-IR methods. Samples from patients (n=50) with candidiasis were collected and identified through culture media. ERG11 gene overexpression was related to resistance against azole-bearing drugs. The antibiogram, well diffusion assay, MICs, and MFCs tests were operated. PCR and Real-time evaluated the expression of the ERG11 gene, and the rate of cell death was detected using Flow Cytometry.

Results: Our data manifested that this novel IL (Ionic Liquid) can inhibit C. albican's growth, reduce the expression of ERG11 and increase dead cells.

Conclusion: The newly synthesized IL had an inhibiting effect on the growth of the C. albicans strains and may be used as an alternative candidate for novel drug design.

查看原文本刊更多论文

对白色念珠菌具有抗真菌活性的新型咪唑基离子液体的合成。

背景和目的：白色念珠菌是免疫力低下患者的一种常见病，由于微生物具有抗药性，因此无法快速治疗。本研究旨在探讨一种新型离子液体（IL）作为新药对白色念珠菌菌株的影响：合成了七种新的二元离子液体混合物，并选择了其中的（[pro-HCl][1-甲基咪唑鎓 3-磺酸盐]（[pro-HCl][MImS]），用 1HNMR、13C NMR 和 FT-IR 方法对其进行了表征。收集了念珠菌病患者（50 人）的样本，并通过培养基进行鉴定。ERG11基因过表达与对含唑类药物的耐药性有关。进行了抗生素图谱、井扩散试验、MICs 和 MFCs 试验。PCR和实时分析评估了ERG11基因的表达，并使用流式细胞仪检测了细胞的死亡率：结果：我们的数据表明，这种新型离子液体（IL）能抑制白僵菌的生长，降低ERG11的表达，增加死亡细胞：结论：新合成的离子液体具有抑制白僵菌菌株生长的作用，可作为新药设计的候选药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.