{"title":"Specific detection of Pyricularia oryzae pathotype Triticum using qPCR and LAMP methods","authors":"Ken-ichi Ikeda, Kaichi Uchihashi, Itsuki Okuda, Zikai Xiang, Hitoshi Nakayashiki","doi":"10.1007/s10327-023-01162-0","DOIUrl":null,"url":null,"abstract":"<p>Wheat blast is an important disease that affects cross-border food security. The causal pathogen, <i>Pyricularia oryzae</i> (syn. <i>Magnaporthe oryzae</i>) <i>Triticum</i> pathotype (MoT), forms a phylogenetic group distinct from the other <i>P. oryzae</i> pathotypes pathogenic on various gramineous plants. Here we developed MoT-specific primer sets for qPCR and LAMP detection methods, and their amplification specificity and efficiency were tested in comparison with previous reported MoT-specific primers. The amplification efficiency and specificity were affected by the polymerase premix. To evaluate the detection specificity, purified DNA samples of 15 MoT isolates, 89 non-MoT isolates of <i>Pyricularia</i> spp., and crude DNA from wheat plants inoculated with MoT were used. We conclude that the Sp3 primer sets for qPCR and the MoT3<i>L</i> primer sets for LAMP were the best in this research.</p>","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"72 1","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of General Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10327-023-01162-0","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Wheat blast is an important disease that affects cross-border food security. The causal pathogen, Pyricularia oryzae (syn. Magnaporthe oryzae) Triticum pathotype (MoT), forms a phylogenetic group distinct from the other P. oryzae pathotypes pathogenic on various gramineous plants. Here we developed MoT-specific primer sets for qPCR and LAMP detection methods, and their amplification specificity and efficiency were tested in comparison with previous reported MoT-specific primers. The amplification efficiency and specificity were affected by the polymerase premix. To evaluate the detection specificity, purified DNA samples of 15 MoT isolates, 89 non-MoT isolates of Pyricularia spp., and crude DNA from wheat plants inoculated with MoT were used. We conclude that the Sp3 primer sets for qPCR and the MoT3L primer sets for LAMP were the best in this research.
期刊介绍：
The Journal of General Plant Pathology welcomes all manuscripts dealing with plant diseases or their control, including pathogen characterization, identification of pathogens, disease physiology and biochemistry, molecular biology, morphology and ultrastructure, genetics, disease transmission, ecology and epidemiology, chemical and biological control, disease assessment, and other topics relevant to plant pathological disorders.
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