Impact of Sleep Deprivation on the Brain's Inflammatory Response Triggered by Lipopolysaccharide and Its Consequences on Spatial Learning and Memory and Long-Term Potentiation in Male Rats.

IF 2.2 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Neuroimmunomodulation Pub Date : 2024-01-01 Epub Date: 2023-12-27 DOI:10.1159/000535784

Maryam Salari, Khadijeh Esmaeilpour, Lily Mohammadipoor-Ghasemabad, Farahnaz Taheri, Mahmoud Hosseini, Vahid Sheibani

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引用次数: 0

Abstract

Introduction: Both sleep deprivation (SD) and inflammation can negatively affect cognitive function. This study aimed to investigate how SD impacts the brain's inflammatory response to lipopolysaccharide (LPS) and its subsequent effects on cognitive functions.

Methods: To this end, male rats were tested through a Morris water maze (MWM) to assess their spatial learning and memory. Also, in vivo field potential recordings (to evaluate synaptic plasticity) were done in the Saline, SD, LPS1 (1 mg/kg/7 days), and LPS1+SD groups. Cytokine levels were measured using an enzyme-linked immunosorbent assay (ELISA).

Results: Based on the results, the LPS1+SD group showed increased total distance and escape latency compared to the other groups in the MWM test. Besides, the LPS1+SD group exhibited a significant decrease in long-term potentiation (LTP) induction and maintenance in the CA1 area of the brain. Finally, the inflammatory cytokine interleukin-1β (IL-1β) levels were significantly higher in the LPS1+SD group than in the Saline group.

Conclusion: These findings suggest that the combined effects of SD and brain inflammatory response can have more harmful effects on cognitive function, LTP, and inflammatory factors than either SD or LPS1 alone.

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剥夺睡眠对脂多糖引发的大脑炎症反应的影响及其对雄性大鼠空间学习记忆和长期潜能的影响

简介睡眠不足（SD）和炎症都会对认知功能产生负面影响。本研究旨在探讨睡眠不足如何影响大脑对脂多糖（LPS）的炎症反应及其对认知功能的影响：为此，研究人员对雄性大鼠进行了莫里斯水迷宫（MWM）测试，以评估它们的空间学习和记忆能力。此外，还在盐水组、SD 组、LPS1（1 毫克/千克/7 天）组和 LPS1+SD 组进行了体内场电位记录（以评估突触可塑性）。使用酶联免疫吸附试验（ELISA）测量细胞因子水平：结果：在MWM测试中，LPS1+SD组的总距离和逃逸潜伏期都比其他组有所增加。此外，LPS1+SD组大脑CA1区的长期电位（LTP）诱导和维持能力显著下降。最后，LPS1+SD 组的炎症细胞因子白细胞介素-1β（IL-1β）水平明显高于生理盐水组：这些研究结果表明，SD 和脑部炎症反应的共同作用对认知功能、LTP 和炎症因子的危害比单独使用 SD 或 LPS1 更严重。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroimmunomodulation 医学-免疫学

CiteScore

3.60

自引率

4.20%

发文量

审稿时长

>12 weeks

期刊介绍： The rapidly expanding area of research known as neuroimmunomodulation explores the way in which the nervous system interacts with the immune system via neural, hormonal, and paracrine actions. Encompassing both basic and clinical research, ''Neuroimmunomodulation'' reports on all aspects of these interactions. Basic investigations consider all neural and humoral networks from molecular genetics through cell regulation to integrative systems of the body. The journal also aims to clarify the basic mechanisms involved in the pathogenesis of the CNS pathology in AIDS patients and in various neurodegenerative diseases. Although primarily devoted to research articles, timely reviews are published on a regular basis.