[Melatonin reverses oxidative damage in the submandibular gland of rats treated with Cyclophosphamide].

Q3 Medicine

Revista de la Facultad de Ciencias Medicas de Cordoba Pub Date : 2023-12-26 DOI:10.31053/1853.0605.v80.n4.40930

Evelin Bachmeier, Fernando Martin Wietz, Daniela Josefina Porta, Lorena Moine, Claudio Gastón Dubersarsky, Catalina Melchora Francia, Maria Elena Samar, Maria Angelica Rivoira, Marcelo Adrian Mazzeo

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引用次数: 0

Abstract

Objetive: Cyclophosphamide (Cf) produces oxidative damage in rat submandibular gland (GSM). In the present work we evaluated the antioxidant protective effect of melatonin (MLT) in GSM of rats treated with Cf.

Methods: 40 adult male Wistar rats were divided into 5 groups (G): G1: control; G2: Control+Ethanol: treated with 1% ethanol for 10 consecutive days. On days 11 and 12 they received a dose of saline; G3: Cf: treated with 1% ethanol for 12 days, days 11 and 12 they received an intraperitoneal (i.p.) dose of Cf 50 mg/Kg/kg of saline. ) of Cf 50 mg/kg bw; G4: Cf + MLT: MLT (5 mg/kg bw, intraperitoneal, dissolved in 1% ethanol) was administered daily, days 11 and 12 received Cf same as G3; G5: MLT: treated 12 consecutive days with MLT (same dose as G4). After 12 hours of fasting, animals were anesthetized to obtain both submandibular glands, then they were sacrificed. Uric acid (UA), lipid peroxides (LPs), aqueous peroxides (APs) and superoxide dismutase (SOD) activity were measured in submandibular gland homogenate. Statistical analysis: we used ANOVA and Bonferroni test pos hoc, considering significant p<0.05.

Results: Cf treatment decreased AU concentration and SOD activity (AU, mg/mg prot., G1: 2.50±0.68; G2: 2.18±0.13; G3: 0.54±0.09* G4: 1.95±0.24#, G5: 2.64±0.47, *p<0.01 G3 vs G1, G2, G4; #p<0.01 G4 vs G3 and G5; SOD, U/mg prot, G1: 4.57±0.95, G2: 4.79±0.94, G3: 2.18±0.53*, G4: 5.13±1.10, G5: 5.09±0.39, *p< 0.01 G3 vs G1, G2, G4 and G5). MLT treatment prevented these effects. In addition, Cf increased PL and PA formation.

Conclusion: MLT improved the redox status in GSM of Cf-treated rats. MLT could prevent oxidative processes in GSM produced by Cf.

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褪黑素可逆转环磷酰胺治疗大鼠下颌下腺的氧化损伤。

目的：环磷酰胺（Cf）会对大鼠的颌下腺（SMG）造成氧化损伤。在本研究中，我们评估了褪黑素（MLT）在接受 Cf 治疗的大鼠颌下腺中的抗氧化保护作用。方法：将 40 只成年雄性 Wistar 大鼠分为 5 组（G）：G1：对照组；G2：对照组+乙醇组：连续 10 天接受 1%乙醇治疗。G3：Cf：用 1%乙醇处理 12 天，第 11 和 12 天腹腔注射（i.p. ）Cf 50 毫克/千克/千克生理盐水。G3：Cf + MLT：每天腹腔注射 MLT（5 毫克/千克体重，溶于 1%乙醇），第 11 和 12 天注射 Cf，剂量与 G3 相同；G5：MLT：连续 12 天注射 MLT（剂量与 G4 相同）。对动物进行麻醉，禁食 24 小时后取出两只 GSM 并将其处死。测定 GSM 匀浆中的尿酸 (UA)、脂质过氧化物 (LPs) 和水过氧化物 (APs) 以及超氧化物歧化酶 (SOD) 活性。统计分析：方差分析和 Bonferroni 检验，考虑显著的 pResults：Cf 处理降低了 AU 浓度和 SOD 活性（AU，mg/mg prot、G1: 2.50±0.68; G2: 2.18±0.13; G3: 0.54±0.09* G4: 1.95±0.24#, G5: 2.64±0.47, *p< 0.01 G3 vs G1, G2, G4; #p< 0.01 G4 vs G3 and G5; SOD, U/mg prot、G1：4.57±0.95；G2：4.79±0.94；G3：2.18±0.53*；G4：5.13±1.10；G5：5.09±0.39；*p< 0.01 G3 vs G1、G2、G4 和 G5）。MLT 处理阻止了这些影响。结论：MLT 可改善 Cf 处理大鼠 GSM 中的氧化还原状态。 MLT 可防止 Cf 在 GSM 中产生氧化过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista de la Facultad de Ciencias Medicas de Cordoba Medicine-Medicine (all)

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： The Journal of the Faculty of Medical Sciences is a scientific publication of the Secretariat of Science and Technology of the Faculty of Medical Sciences of the National University of Cordoba. Its objective is to disseminate and promote research work related to Medical and Biological Sciences. It publishes scientific works of national and international professionals on different topics related to health sciences from the field of medicine, nursing, kinesiology, diagnostic imaging, phonoaudiology, nutrition, public health, chemical sciences, dentistry and related.