Serum amyloid A gene expression and AA amyloid formation in A/J and SJL/J mice.

H Rokita, T Shirahama, A S Cohen, J D Sipe
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Abstract

Serum amyloid A (SAA) gene expression and AA amyloid fibril formation were studied in A/J and SJL/J mice, two strains which have been reported to possess defects in AA fibril formation. Four types of inflammatory stimulation were employed: acute inflammation stimulated with lipopolysaccharide (LPS), chronic inflammation with casein in complete Freund's adjuvant, amyloidosis with injection of amyloid enhancing factor (AEF) together with casein in complete Freund's adjuvant, and non-amyloidogenic inflammation in the presence of AEF with injection of AEF together with LPS. Both A/J and SJL/J mice developed splenic amyloidosis 1 day after initiation of chronic inflammation in the presence of AEF. No amyloid deposits were detected during any of the other types of inflammation. Amyloidotic mice exhibited decreased amounts of SAA mRNA in liver and spleen concomitant with decreased amounts of SAA in serum. Alpha-I-acid glycoprotein mRNA was present in liver throughout the course of AEF accelerated amyloidosis, indicating that decreased SAA gene expression and AA fibril formation is not part of a general inhibitory effect of AEF on protein synthesis.

A/J和SJL/J小鼠血清淀粉样蛋白A基因表达和AA淀粉样蛋白形成。
研究了A/J和SJL/J小鼠血清淀粉样蛋白A (SAA)基因的表达和AA淀粉样蛋白纤维的形成,这两种小鼠在AA纤维形成方面存在缺陷。采用四种类型的炎症刺激:脂多糖(LPS)刺激的急性炎症,完全弗氏佐剂中酪蛋白引起的慢性炎症,完全弗氏佐剂中注射淀粉样蛋白增强因子(AEF)和酪蛋白引起的淀粉样变性,AEF和LPS同时注射AEF引起的非淀粉样变性炎症。A/J和SJL/J小鼠在AEF存在下的慢性炎症开始1天后发生脾淀粉样变性。在其他类型的炎症中未检测到淀粉样蛋白沉积。淀粉样变小鼠肝脏和脾脏中SAA mRNA含量降低,血清中SAA含量降低。在AEF加速淀粉样变的整个过程中,肝脏中都存在α - i -酸糖蛋白mRNA,这表明SAA基因表达的减少和AA纤维的形成并不是AEF对蛋白质合成的普遍抑制作用的一部分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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