Spatial Variations in the Nasal Microbiota of Staff Working in a Healthcare-Associated Research Core Facility.

IF 2.9 3区医学 Q1 MEDICINE, GENERAL & INTERNAL

Medical Principles and Practice Pub Date : 2024-01-01 Epub Date: 2023-12-26 DOI:10.1159/000535983

Abu Salim Mustafa, Nazima Habibi

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引用次数: 0

Abstract

Objective: Workers in the healthcare sector are exposed to a multitude of bacterial genera. The location of their work contributes significantly to shaping personal microbiomes. In this study, we investigated the role of the workspace on the nasal bacteriome of staff working in a healthcare-associated research facility.

Methods: The anterior nares of 10 staff working in different laboratories on the ground and first floor of the research facility were aseptically swabbed. Genomic DNA from each sample was used to amplify the V3 and V4 regions of the 16S rRNA gene. The amplified products were sequenced using the MiSeq sequencer (Illumina). Operational taxonomic units were filtered through MG-RAST v.3.6. Taxonomic profiling and visualizations were performed in MicrobiomeAnalyst v2.0.

Results: The Wilcoxson Sum test at median abundances (p < 0.05) indicated that seven taxa (Micromonosporaceae, Micromonospora, Lactobacillaceae, Lactobacillus, Betaproteobacteria, Burkholderiales, Pectobacterium) were significantly diverse between ground-floor and first-floor workers. The analysis of similarity coefficient was 0.412 (p < 0.03) between the ground and the first-floor workers. Random forest analysis predicted 15 features that were significantly different (p < 0.05) in individuals working in different laboratories. Species richness and evenness also differed according to the placement of individuals in respective laboratories.

Conclusion: These findings add to the knowledge that the healthcare support staff are at a speculated occupational risk. A slight shift in the abundances of bacterial genera and species might lead to unwanted consequences. Continual monitoring is thus warranted.

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在医疗相关研究核心机构工作的员工鼻腔微生物群的空间变化。

目的：医疗保健行业的工作人员会接触到多种细菌属。他们的工作地点在很大程度上影响着个人微生物组的形成。在这项研究中，我们调查了工作空间对医疗保健相关研究机构工作人员鼻腔细菌组的影响：方法：我们对在研究机构一楼和二楼不同实验室工作的 10 名员工的前鼻腔进行了无菌拭抹。用每个样本的基因组 DNA 扩增 16S rRNA 基因的 V3 和 V4 区域。扩增产物使用 MiSeq 测序仪（Illumina）进行测序。通过 MG-RAST v 3.6 过滤操作分类单元（OTU）。分类剖析和可视化在 MicrobiomeAnalyst v2.0.Results 中进行：根据丰度中位数进行的 Wilcoxson Sum 检验（p < 0.05）表明，7 个分类群（小孢子菌科、小孢子菌属、乳酸菌科、乳酸杆菌属、贝特蛋白菌属、伯克霍尔德氏菌属、果胶杆菌属）在一楼和二楼工人之间存在显著差异。地面和一楼工人之间的相似性分析（ANOSIM）系数为 0.412（P < 0.03）。随机森林分析预测在不同实验室工作的个体有 15 个显著不同的特征（p < 0.05）。物种丰富度和均匀度也因个人在不同实验室的工作地点而有所不同：这些发现使人们进一步认识到，医疗保健辅助人员可能存在职业风险。细菌属种数量的轻微变化可能会导致不必要的后果。因此，有必要进行持续监测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medical Principles and Practice 医学-医学：内科

CiteScore

6.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Medical Principles and Practice'', as the journal of the Health Sciences Centre, Kuwait University, aims to be a publication of international repute that will be a medium for dissemination and exchange of scientific knowledge in the health sciences.