Transferosomes stabilized hydrogel incorporated rhodomyrtone-rich extract from Rhodomyrtus tomentosa leaf fortified with phosphatidylcholine for the management of skin and soft-tissue infections.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2024-02-01 Epub Date: 2023-12-27 DOI:10.1007/s10529-023-03452-1
Julalak Chorachoo Ontong, Sudarshan Singh, Thanyaluck Siriyong, Supayang P Voravuthikunchai
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Abstract

Rhodomyrtus tomentosa leaf (RT)-incorporated transferosomes were developed with lecithin and cholesterol blends with edge activators at different ratios. RT-transferosomes were characterized and employed in transferosomal gel formulations for the management of skin and soft-tissue infections. The optimized formulation entrapped up to 81.90 ± 0.31% of RT with spherical vesicles (405.3 ± 2.0 nm), polydispersity index value of 0.16 ± 0.08, and zeta potential of - 61.62 ± 0.86 mV. Total phenolic and flavonoid contents of RT-transferosomes were 15.65 ± 0.04 μg GAE/g extract and 43.13 ± 0.91 μg QE/g extract, respectively. RT-transferosomes demonstrated minimum inhibitory and minimum bactericidal concentrations at 8-256 and 64-1024 μg/mL, respectively. Free radical scavenging assay showed RT-transferosomes with high scavenging activity against DPPH and ABTS radicals. Moreover, RT-transferosomes demonstrated moderate activity against mushroom tyrosinase, with IC50 values of 245.32 ± 1.32 μg/mL. The biocompatibility results against L929 fibroblast and Vero cells demonstrated IC50 at 7.05 ± 0.17 and 4.73 ± 0.13 μg/mL, respectively. In addition, nitric oxide production significantly decreased by 6.78-88.25% following the treatment with 31.2-500 ng/mL RT-transferosomes (p < 0.001). Furthermore, the freeze-thaw stability study displayed no significant change in stability in the sedimentation and pH of gel fortified with RT-transferosomes. The results suggested that RT-transferosome formulation can be effectively employed as natural biomedicines for scar prevention and the management of skin soft-tissue infections.

Abstract Image

转运体稳定水凝胶含有富含磷脂酰胆碱的红景天叶提取物,可用于治疗皮肤和软组织感染。
利用卵磷脂和胆固醇混合物以及不同比例的边缘活化剂,开发了红景天叶(RT)结合转移体。对 RT 转运体进行了表征,并将其用于治疗皮肤和软组织感染的转运体凝胶配方中。优化后的配方可夹带高达 81.90 ± 0.31% 的 RT,其囊泡呈球形(405.3 ± 2.0 nm),多分散指数值为 0.16 ± 0.08,zeta 电位为 - 61.62 ± 0.86 mV。RT 转化体的总酚和类黄酮含量分别为 15.65 ± 0.04 μg GAE/g 提取物和 43.13 ± 0.91 μg QE/g 提取物。RT 转化体的最低抑菌浓度和最低杀菌浓度分别为 8-256 μg/mL 和 64-1024 μg/mL。自由基清除试验表明,RT-转运体对 DPPH 和 ABTS 自由基具有很高的清除活性。此外,RT-转移体对蘑菇酪氨酸酶具有中等活性,IC50 值为 245.32 ± 1.32 μg/mL。对 L929 成纤维细胞和 Vero 细胞的生物相容性结果显示,IC50 值分别为 7.05 ± 0.17 和 4.73 ± 0.13 μg/mL。此外,用 31.2-500 纳克/毫升 RT-转运体处理后,一氧化氮的产生量明显减少了 6.78-88.25% (p
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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