Transferosomes stabilized hydrogel incorporated rhodomyrtone-rich extract from Rhodomyrtus tomentosa leaf fortified with phosphatidylcholine for the management of skin and soft-tissue infections.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2024-02-01 Epub Date: 2023-12-27 DOI:10.1007/s10529-023-03452-1

Julalak Chorachoo Ontong, Sudarshan Singh, Thanyaluck Siriyong, Supayang P Voravuthikunchai

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Abstract

Rhodomyrtus tomentosa leaf (RT)-incorporated transferosomes were developed with lecithin and cholesterol blends with edge activators at different ratios. RT-transferosomes were characterized and employed in transferosomal gel formulations for the management of skin and soft-tissue infections. The optimized formulation entrapped up to 81.90 ± 0.31% of RT with spherical vesicles (405.3 ± 2.0 nm), polydispersity index value of 0.16 ± 0.08, and zeta potential of - 61.62 ± 0.86 mV. Total phenolic and flavonoid contents of RT-transferosomes were 15.65 ± 0.04 μg GAE/g extract and 43.13 ± 0.91 μg QE/g extract, respectively. RT-transferosomes demonstrated minimum inhibitory and minimum bactericidal concentrations at 8-256 and 64-1024 μg/mL, respectively. Free radical scavenging assay showed RT-transferosomes with high scavenging activity against DPPH and ABTS radicals. Moreover, RT-transferosomes demonstrated moderate activity against mushroom tyrosinase, with IC50 values of 245.32 ± 1.32 μg/mL. The biocompatibility results against L929 fibroblast and Vero cells demonstrated IC₅₀ at 7.05 ± 0.17 and 4.73 ± 0.13 μg/mL, respectively. In addition, nitric oxide production significantly decreased by 6.78-88.25% following the treatment with 31.2-500 ng/mL RT-transferosomes (p < 0.001). Furthermore, the freeze-thaw stability study displayed no significant change in stability in the sedimentation and pH of gel fortified with RT-transferosomes. The results suggested that RT-transferosome formulation can be effectively employed as natural biomedicines for scar prevention and the management of skin soft-tissue infections.

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转运体稳定水凝胶含有富含磷脂酰胆碱的红景天叶提取物，可用于治疗皮肤和软组织感染。

利用卵磷脂和胆固醇混合物以及不同比例的边缘活化剂，开发了红景天叶（RT）结合转移体。对 RT 转运体进行了表征，并将其用于治疗皮肤和软组织感染的转运体凝胶配方中。优化后的配方可夹带高达 81.90 ± 0.31% 的 RT，其囊泡呈球形（405.3 ± 2.0 nm），多分散指数值为 0.16 ± 0.08，zeta 电位为 - 61.62 ± 0.86 mV。RT 转化体的总酚和类黄酮含量分别为 15.65 ± 0.04 μg GAE/g 提取物和 43.13 ± 0.91 μg QE/g 提取物。RT 转化体的最低抑菌浓度和最低杀菌浓度分别为 8-256 μg/mL 和 64-1024 μg/mL。自由基清除试验表明，RT-转运体对 DPPH 和 ABTS 自由基具有很高的清除活性。此外，RT-转移体对蘑菇酪氨酸酶具有中等活性，IC50 值为 245.32 ± 1.32 μg/mL。对 L929 成纤维细胞和 Vero 细胞的生物相容性结果显示，IC50 值分别为 7.05 ± 0.17 和 4.73 ± 0.13 μg/mL。此外，用 31.2-500 纳克/毫升 RT-转运体处理后，一氧化氮的产生量明显减少了 6.78-88.25% （p

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.