Exploring the impact of silica and silica-based nanoparticles on serological parameters, histopathology, organ toxicity, and genotoxicity in Rattus norvegicus.

IF 7.5 Q1 CHEMISTRY, PHYSICAL
Arooj Ali , Saba Saeed , Riaz Hussain , Muhammad Saqib Saif , Muhammad Waqas , Iqra Asghar , Xuang Xue , Murtaza Hasan
{"title":"Exploring the impact of silica and silica-based nanoparticles on serological parameters, histopathology, organ toxicity, and genotoxicity in Rattus norvegicus.","authors":"Arooj Ali ,&nbsp;Saba Saeed ,&nbsp;Riaz Hussain ,&nbsp;Muhammad Saqib Saif ,&nbsp;Muhammad Waqas ,&nbsp;Iqra Asghar ,&nbsp;Xuang Xue ,&nbsp;Murtaza Hasan","doi":"10.1016/j.apsadv.2023.100551","DOIUrl":null,"url":null,"abstract":"<div><p>Continuous exposure to nanoparticles (NPs) poses potential hazards to human health and animal life, necessitating thorough monitoring and investigation of environmental contaminants to mitigate their adverse effects. Therefore, this study investigates the histopathological alterations, serum biochemistry, oxidative stress biomarkers, and genotoxicity endpoints induced by crystalline silica (C-SiO<sub>2</sub>) NPs, sliver-silica (Ag-SiO<sub>2</sub>) and zinc-oxide silica (ZnO-SiO<sub>2</sub>) NPs in <em>Rattus Norvegicus</em>. Twenty-five rats were blindly distributed into groups (A, B, C, &amp; D), with B, C, and D administered 500 µg/kg of ZnO-SiO<sub>2</sub>, Ag-SiO<sub>2</sub>, and C-SiO<sub>2</sub> NPs intravenously (IV), while A served as the control. Severe histopathological alterations, including widening of urinary spaces, edema, necrosis of neurons, atrophy of neurons, myofibrillolysis, inflammatory exudate, disorganization of splenic cells, and pyknotic nuclei, were observed in C-SiO<sub>2</sub> NPs-exposed rats across study organs. Compared to controls, C-SiO<sub>2</sub> NPs significantly altered 95 % of serological, DNA damage, histopathological, and oxidative stress parameters. Ag-SiO<sub>2</sub> and ZnO-SiO<sub>2</sub> NPs affected 75 % and 60 % of these parameters, respectively. Among the NPs, C-SiO<sub>2</sub> exhibited the most adverse effects. The study concludes that SiO<sub>2</sub> coating on metal (Ag) and metal oxide (ZnO) rendered these substances biocompatible, suggesting potential applications for reducing adverse effects in living organisms. These findings also contribute to the genotoxic profiling of NPs and their potential health hazards upon exposure to humans.</p></div>","PeriodicalId":34303,"journal":{"name":"Applied Surface Science Advances","volume":null,"pages":null},"PeriodicalIF":7.5000,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266652392300185X/pdfft?md5=6f2db19c78abb7a6a034851f4436f06b&pid=1-s2.0-S266652392300185X-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Surface Science Advances","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S266652392300185X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Continuous exposure to nanoparticles (NPs) poses potential hazards to human health and animal life, necessitating thorough monitoring and investigation of environmental contaminants to mitigate their adverse effects. Therefore, this study investigates the histopathological alterations, serum biochemistry, oxidative stress biomarkers, and genotoxicity endpoints induced by crystalline silica (C-SiO2) NPs, sliver-silica (Ag-SiO2) and zinc-oxide silica (ZnO-SiO2) NPs in Rattus Norvegicus. Twenty-five rats were blindly distributed into groups (A, B, C, & D), with B, C, and D administered 500 µg/kg of ZnO-SiO2, Ag-SiO2, and C-SiO2 NPs intravenously (IV), while A served as the control. Severe histopathological alterations, including widening of urinary spaces, edema, necrosis of neurons, atrophy of neurons, myofibrillolysis, inflammatory exudate, disorganization of splenic cells, and pyknotic nuclei, were observed in C-SiO2 NPs-exposed rats across study organs. Compared to controls, C-SiO2 NPs significantly altered 95 % of serological, DNA damage, histopathological, and oxidative stress parameters. Ag-SiO2 and ZnO-SiO2 NPs affected 75 % and 60 % of these parameters, respectively. Among the NPs, C-SiO2 exhibited the most adverse effects. The study concludes that SiO2 coating on metal (Ag) and metal oxide (ZnO) rendered these substances biocompatible, suggesting potential applications for reducing adverse effects in living organisms. These findings also contribute to the genotoxic profiling of NPs and their potential health hazards upon exposure to humans.

探讨二氧化硅和二氧化硅基纳米粒子对鼠类血清学参数、组织病理学、器官毒性和遗传毒性的影响。
持续暴露于纳米粒子(NPs)会对人类健康和动物生命造成潜在危害,因此有必要对环境污染物进行全面监测和调查,以减轻其不利影响。因此,本研究调查了结晶二氧化硅(C-SiO2)NPs、片状二氧化硅(Ag-SiO2)和氧化锌二氧化硅(ZnO-SiO2)NPs诱导的大鼠组织病理学改变、血清生物化学、氧化应激生物标志物和遗传毒性终点。将 25 只大鼠盲法分组(A、B、C、& D),B、C 和 D 组静脉注射 ZnO-SiO2、Ag-SiO2 和 C-SiO2 NPs 500 µg/kg,A 组为对照组。暴露于 C-SiO2 NPs 的大鼠各研究器官均出现了严重的组织病理学改变,包括尿道间隙增宽、水肿、神经元坏死、神经元萎缩、肌纤维溶解、炎性渗出物、脾脏细胞紊乱和细胞核坏死。与对照组相比,C-二氧化硅氮氧化物显著改变了 95% 的血清学、DNA 损伤、组织病理学和氧化应激参数。Ag-SiO2和ZnO-SiO2纳米粒子分别影响了75%和60%的这些参数。在这些 NPs 中,C-SiO2 的不良影响最大。研究得出结论,金属(Ag)和金属氧化物(ZnO)上的二氧化硅涂层使这些物质具有生物相容性,这表明它们在减少对生物体的不利影响方面具有潜在的应用价值。这些研究结果还有助于分析氮氧化物的遗传毒性及其接触人体后对健康的潜在危害。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
8.10
自引率
1.60%
发文量
128
审稿时长
66 days
期刊介绍:
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信