{"title":"Electron irradiation of zein protein-loaded nano CaO2/CD for enhancing infectious diabetic wounds with adaptive hydrophobicity-to-hydrophilicity","authors":"Lenian Zhou, Shang Guo, Zhenyou Dong, Pei Liu, Wenyan Shi, Longxiang Shen, Junhui Yin","doi":"10.1016/j.mtadv.2023.100458","DOIUrl":null,"url":null,"abstract":"<p>Chronic diabetic cutaneous wounds resulting from inflammatory conditions present an ongoing challenge for current therapies and impose a significant burden on individuals with diabetes, impacting their quality of life. Infection-related diabetic skin wounds require dry conditions to inhibit bacterial growth. However, as the wounds progress, moisture becomes necessary to facilitate the healing process. In this study, we propose a novel therapeutic strategy for diabetic skin repair by creating bio-dressings with adjustable “hydrophobic” and “hydrophilic” characteristics to accommodate the changing stages of the disease. We developed a skin dressing by loading calcium peroxide (CaO<sub>2</sub>) nanoparticles onto carbon dots (CD)-modified irradiated zein (Ir-Zein). This dressing releases reactive oxygen species (ROS) from CaO<sub>2</sub>, providing antibacterial effects, while the presence of CD enables a sustained release of CaO<sub>2</sub>. The calcium ions produced by CaO<sub>2</sub> degradation further promote skin regeneration. Ir-Zein protein, a cost-effective and easily processed natural plant protein, exhibits excellent biocompatibility. Importantly, in diabetic rats with full-thickness skin defects, the CaO<sub>2</sub>/CD@Ir-Zein film significantly accelerated the healing of chronic wounds. Mechanistic investigations revealed that the film effectively reduced inflammation by inhibiting the polarization of macrophages towards the M1 phenotype and capturing pro-inflammatory cytokines. In summary, our findings demonstrate the effectiveness of the CaO<sub>2</sub>/CD@Ir-Zein film’s “adaptive hydrophobicity-to-hydrophilicity” in promoting the transition of chronic wounds from the inflammatory stage and skin repair. CaO<sub>2</sub>/CD@Ir Zein is a novel bio-dressing that can adapt to the changing environment of infected diabetic skin wound healing.</p>","PeriodicalId":48495,"journal":{"name":"Materials Today Advances","volume":"64 1","pages":""},"PeriodicalIF":8.1000,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materials Today Advances","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1016/j.mtadv.2023.100458","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Chronic diabetic cutaneous wounds resulting from inflammatory conditions present an ongoing challenge for current therapies and impose a significant burden on individuals with diabetes, impacting their quality of life. Infection-related diabetic skin wounds require dry conditions to inhibit bacterial growth. However, as the wounds progress, moisture becomes necessary to facilitate the healing process. In this study, we propose a novel therapeutic strategy for diabetic skin repair by creating bio-dressings with adjustable “hydrophobic” and “hydrophilic” characteristics to accommodate the changing stages of the disease. We developed a skin dressing by loading calcium peroxide (CaO2) nanoparticles onto carbon dots (CD)-modified irradiated zein (Ir-Zein). This dressing releases reactive oxygen species (ROS) from CaO2, providing antibacterial effects, while the presence of CD enables a sustained release of CaO2. The calcium ions produced by CaO2 degradation further promote skin regeneration. Ir-Zein protein, a cost-effective and easily processed natural plant protein, exhibits excellent biocompatibility. Importantly, in diabetic rats with full-thickness skin defects, the CaO2/CD@Ir-Zein film significantly accelerated the healing of chronic wounds. Mechanistic investigations revealed that the film effectively reduced inflammation by inhibiting the polarization of macrophages towards the M1 phenotype and capturing pro-inflammatory cytokines. In summary, our findings demonstrate the effectiveness of the CaO2/CD@Ir-Zein film’s “adaptive hydrophobicity-to-hydrophilicity” in promoting the transition of chronic wounds from the inflammatory stage and skin repair. CaO2/CD@Ir Zein is a novel bio-dressing that can adapt to the changing environment of infected diabetic skin wound healing.
期刊介绍:
Materials Today Advances is a multi-disciplinary, open access journal that aims to connect different communities within materials science. It covers all aspects of materials science and related disciplines, including fundamental and applied research. The focus is on studies with broad impact that can cross traditional subject boundaries. The journal welcomes the submissions of articles at the forefront of materials science, advancing the field. It is part of the Materials Today family and offers authors rigorous peer review, rapid decisions, and high visibility.