Time-series bioinformatics analysis of SARS-CoV-infected cells to identify the biological processes associated with severe acute respiratory syndrome.

Q3 Medicine

Human Antibodies Pub Date : 2023-01-01 DOI:10.3233/HAB-230012

Razieh Fatehi, Farinaz Khosravian, Mansoor Salehi, Mohammad Kazemi

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引用次数: 0

Abstract

Background: The COVID-19 pandemic, caused by the new virus of the coronavirus family, SARS-CoV-2, could lead to acute respiratory syndrome. The molecular mechanisms related to this disorder are still debatable.

Methods: In this study to understand the pathogenicity mechanism of SARS-CoV-2, using the bioinformatics approaches, we investigated the expression of involved genes, their regulatory, and main signaling pathways during the time on days 1, 2, 3, and 4 of SARS-CoV infected cells.

Results: Here, our investigation shows the complex changes in gene expression on days 2 and 3 post-infection. The functional analysis showed that especially related to immune response, response to other organisms, and defense response. IL6-AS1 is the predicted long non-coding RNA and is a key regulator during infection. In this study, for the first time has been reported the role of IL6-AS1. Also, the correlation of differential expression genes with the level of immune infiltration was shown in the relationship of Natural killer cells and T cell CD 4+ with DE genes.

Conclusion: In the current study, identification of the altered expression pattern of genes in SARS-CoV-infected cells in time course also can help identify and link the molecular mechanisms and explore the holistic view of infection of SARS-CoV-2.

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对 SARS-CoV 感染细胞进行时间序列生物信息学分析，以确定与严重急性呼吸系统综合征相关的生物过程。

背景：由冠状病毒家族的新病毒SARS-CoV-2引起的COVID-19大流行可能导致急性呼吸综合征。方法：本研究旨在了解 SARS-CoV-2 的致病机制：为了了解 SARS-CoV-2 的致病机制，本研究利用生物信息学方法研究了 SARS-CoV 感染细胞第 1、2、3 和 4 天期间相关基因的表达及其调控和主要信号通路：结果：我们的研究表明，在感染后的第 2 天和第 3 天，基因表达发生了复杂的变化。功能分析显示，这些基因尤其与免疫反应、对其他生物的反应和防御反应有关。IL6-AS1 是预测的长非编码 RNA，是感染过程中的关键调控因子。本研究首次报道了 IL6-AS1 的作用。此外，在自然杀伤细胞和 T 细胞 CD 4+ 与 DE 基因的关系中，还显示了差异表达基因与免疫浸润水平的相关性：在本研究中，识别 SARS-CoV 感染细胞中基因在时间进程中的表达模式改变也有助于识别和联系分子机制，探索 SARS-CoV-2 感染的整体观点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Antibodies Medicine-Immunology and Allergy

CiteScore

3.50

自引率

0.00%

发文量

期刊介绍： Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology under a single, cohesive theme. This includes fundamental research, applied science and clinical applications. Emphasis in the published articles is on antisera, monoclonal antibodies, fusion partners, EBV transformation, transfections, in vitro immunization, defined antigens, tissue reactivity, scale-up production, chimeric antibodies, autoimmunity, natural antibodies/immune response, anti-idiotypes, and hybridomas secreting interesting growth factors. Immunoregulatory molecules, including T cell hybridomas, will also be featured.