A. O. Berestetskiy, E. N. Grigoryeva, M. O. Petrova, I. V. Senderskiy, E. A. Stepanycheva
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引用次数: 0
Abstract—Bipolaris sorokiniana is circumglobally widespread root and leaf pathogen of cereals and grasses. It can grow both endo- and epiphytically and also survive in the soil. The chemical mechanisms of such good persistence of the fungus are poorly studied. This study assays the biological (phytotoxic, antimicrobial, insecticidal, and cytotoxic) activity of extracts from cultures of four B. sorokiniana of distinct geographical origin produced on three different media. The composition of media and, to lesser extent, origin of the isolate affected the yield of extractive matter, spectrum, and level of biological activity of extracts. Extracts from liquid culture mycelium demonstrated relatively high insecticidal activity to wheat aphid and galleria, while extracts from solid cultures were highly cytotoxic to the Sf9 cell line. Phytotoxic activity was the most pronounced in extracts from culture liquids. No correlation was found between phytotoxic and insecticidal or cytotoxic activity of extracts indicating possible production of insecticidal metabolites by B. sorokiniana isolates. The revealed insecticidal properties of the fungal metabolites were able to explain its high competition activity for the plant substrate and broaden the biotechnological potential B. sorokiniana of as a producer of bioactive compounds.
Abstract-Bipolaris sorokiniana 是一种在全球广泛传播的谷物和禾本科植物根部和叶片病原体。它既能内生，也能附生，还能在土壤中生存。对这种真菌如此持久的化学机制研究甚少。本研究检测了在三种不同培养基上培养出的四种不同地理来源的 B. sorokiniana 的提取物的生物（植物毒性、抗菌性、杀虫性和细胞毒性）活性。培养基的组成以及分离物的来源在较小程度上影响了提取物的产量、光谱和生物活性水平。从液体培养菌丝体中提取的提取物对小麦蚜虫和五倍子具有较高的杀虫活性，而从固体培养物中提取的提取物对 Sf9 细胞系具有较高的细胞毒性。培养液提取物的植物毒性最为明显。提取物的植物毒性与杀虫或细胞毒性之间没有相关性，这表明 B. sorokiniana 分离物可能产生了杀虫代谢物。所揭示的真菌代谢产物的杀虫特性能够解释其对植物底物的高竞争活性，并拓宽了 B. sorokiniana 作为生物活性化合物生产者的生物技术潜力。
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