Antioxidant Activity of Auricularia auricula Polysaccharides with Different Molecular Weights and Cytotoxicity Difference of Polysaccharides Regulated CaOx to HK-2 Cells

IF 4.7 3区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Bao-Li Heng, Fan-Yu Wu, Jing-Hong Liu, Jian-Ming Ouyang
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引用次数: 0

Abstract

Objective. This study aimed to investigate the growth of calcium oxalate (CaOx) crystals regulated by Auricularia auricular polysaccharides (AAPs) with different viscosity-average molecular weights (), the toxicity of AAP-regulated CaOx crystals toward HK-2 cells, and the prevention and treatment capabilities of AAPs for CaOx stones. Methods. The scavenging capability and reducing capacity of four kinds of AAPs ( of 31.52, 11.82, 5.86, and 3.34 kDa) on hydroxyl, ABTS, and DPPH free radicals and their capability to chelate divalent iron ions were detected. AAP-regulated CaOx crystals were evaluated by using zeta potential, thermogravimetric analysis, X-ray diffraction, and scanning electron microscopy. The cytotoxicity of AAP-regulated crystals was evaluated through examination of cell viability, cell death, malondialdehyde (MDA) content, and cell surface hyaluronic acid (HA) expression. Results. The in vitro antioxidant activities of the four AAPs were observed in the following order: AAP0 < AAP1 < AAP2 < AAP3. Thus, AAP3, which had the smallest , had the strongest antioxidant activity. AAPs can inhibit the growth of CaOx monohydrate (COM), induce the formation of CaOx dihydrate (COD), and reduce the degree of crystal aggregation, with AAP3 exhibiting the strongest capability. Cell experiments showed the lowest cytotoxicity in AAP3-regulated CaOx crystals, along with the lowest MDA content, HA expression, and cell mortality. In addition, COD presented less cytotoxicity than COM. Meanwhile, the cytotoxicity of blunt crystals was less than that of sharp crystals. Conclusion. AAPs, particularly AAP3, showed an excellent antioxidative capability in vitro, and AAP3-regulated CaOx crystals presented minimal cytotoxicity.
不同分子量的黑木耳多糖的抗氧化活性及多糖调节 CaOx 对 HK-2 细胞的细胞毒性差异
研究目的本研究旨在探讨不同粘均分子量()的耳穴多糖(AAPs)对草酸钙(CaOx)结晶生长的调控作用、AAPs调控的CaOx结晶对HK-2细胞的毒性以及AAPs对CaOx结石的预防和治疗作用。研究方法检测四种 AAPs(31.52、11.82、5.86 和 3.34 kDa)对羟基、ABTS 和 DPPH 自由基的清除能力和还原能力及其对二价铁离子的螯合能力。利用ZETA电位、热重分析、X射线衍射和扫描电子显微镜对AAP调控的CaOx晶体进行了评估。通过检测细胞活力、细胞死亡、丙二醛(MDA)含量和细胞表面透明质酸(HA)的表达,评估了 AAP 调节晶体的细胞毒性。结果四种抗氧化剂的体外抗氧化活性按以下顺序排列:AAP0;AAP1;AAP2;AAP3。因此,AAP3 的抗氧化活性最强,而 AAP1 的抗氧化活性最小。AAPs 可以抑制一水 CaOx(COM)的生长,诱导二水 CaOx(COD)的形成,并降低晶体的聚集程度,其中 AAP3 的能力最强。细胞实验显示,AAP3 调节的 CaOx 晶体细胞毒性最低,MDA 含量、HA 表达和细胞死亡率也最低。此外,COD 的细胞毒性低于 COM。同时,钝晶体的细胞毒性低于尖晶体。结论AAPs(尤其是 AAP3)在体外表现出卓越的抗氧化能力,AAP3 调节的 CaOx 晶体的细胞毒性极低。
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来源期刊
Bioinorganic Chemistry and Applications
Bioinorganic Chemistry and Applications 化学-生化与分子生物学
CiteScore
7.00
自引率
5.30%
发文量
105
审稿时长
>12 weeks
期刊介绍: Bioinorganic Chemistry and Applications is primarily devoted to original research papers, but also publishes review articles, editorials, and letter to the editor in the general field of bioinorganic chemistry and its applications. Its scope includes all aspects of bioinorganic chemistry, including bioorganometallic chemistry and applied bioinorganic chemistry. The journal welcomes papers relating to metalloenzymes and model compounds, metal-based drugs, biomaterials, biocatalysis and bioelectronics, metals in biology and medicine, metals toxicology and metals in the environment, metal interactions with biomolecules and spectroscopic applications.
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