Recombinant Cells as a Tool for Evaluating the Specific Activity of Radiolabeled Antibodies against Endoglin (CD105)

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Biochemistry and Microbiology Pub Date : 2023-12-14 DOI:10.1134/S0003683823070049

O. A. Shashkova, I. V. Smirnov, A. A. Pinevich, K. O. Avrov, L. A. Terekhina, I. S. Malakhov, A. Yu. Stolbovaya, I. V. Gryazeva, N. L. Vartanyan, I. Yu. Krutetskaya, D. O. Antuganov, S. V. Shatik, M. P. Samoilovich

{"title":"Recombinant Cells as a Tool for Evaluating the Specific Activity of Radiolabeled Antibodies against Endoglin (CD105)","authors":"O. A. Shashkova, I. V. Smirnov, A. A. Pinevich, K. O. Avrov, L. A. Terekhina, I. S. Malakhov, A. Yu. Stolbovaya, I. V. Gryazeva, N. L. Vartanyan, I. Yu. Krutetskaya, D. O. Antuganov, S. V. Shatik, M. P. Samoilovich","doi":"10.1134/S0003683823070049","DOIUrl":null,"url":null,"abstract":"<div>One of the most important characteristics of radiolabeled antibodies and their derivatives is the size of the immunoreactive fraction. Measuring this parameter requires a high density of target molecules, which is rarely achievable with tumor cells. The solution to the problem of radioimmunoconjugate testing was the creation of recombinant cells carrying human endoglin (CD105). The recipients of the endoglin gene (ENG) were rat C6 glioma cells, which are characterized by ease of cultivation and high transfection efficiency. The obtained C6-ENG cells carried 1.3 × 106 CD105 molecules on the membrane and were used to determine the immunoreactive fraction of 68Ga and 89Zr radiolabeled anti-CD105 monoclonal antibodies and their Fab-fragments. The creation of stable recombinant cell lines for in vitro testing the specific activity of radiolabeled antibodies and their derivatives seems promising for the development of new radiopharmaceuticals.</div>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683823070049","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

One of the most important characteristics of radiolabeled antibodies and their derivatives is the size of the immunoreactive fraction. Measuring this parameter requires a high density of target molecules, which is rarely achievable with tumor cells. The solution to the problem of radioimmunoconjugate testing was the creation of recombinant cells carrying human endoglin (CD105). The recipients of the endoglin gene (ENG) were rat C6 glioma cells, which are characterized by ease of cultivation and high transfection efficiency. The obtained C6-ENG cells carried 1.3 × 10⁶ CD105 molecules on the membrane and were used to determine the immunoreactive fraction of ⁶⁸Ga and ⁸⁹Zr radiolabeled anti-CD105 monoclonal antibodies and their Fab-fragments. The creation of stable recombinant cell lines for in vitro testing the specific activity of radiolabeled antibodies and their derivatives seems promising for the development of new radiopharmaceuticals.

Abstract Image

查看原文本刊更多论文

以重组细胞为工具评估针对内切蛋白（CD105）的放射性标记抗体的特异性活性

放射性标记抗体及其衍生物最重要的特征之一是免疫反应部分的大小。测量这一参数需要高密度的靶分子，而肿瘤细胞很少能做到这一点。解决放射免疫结合剂测试问题的方法是创建携带人内凝集素（CD105）的重组细胞。内切蛋白基因（ENG）的受体是大鼠 C6 胶质瘤细胞，其特点是易于培养且转染效率高。获得的 C6-ENG 细胞膜上携带有 1.3 × 106 个 CD105 分子，可用于测定 68Ga 和 89Zr 放射性标记的抗 CD105 单克隆抗体及其 Fab 片段的免疫反应部分。建立稳定的重组细胞系用于体外测试放射性标记抗体及其衍生物的特异性活性，对于开发新的放射性药物似乎大有可为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.