Biological synthesis and characterization of antibacterial manganese oxide nanoparticles using Bacillus subtilis ATCC6633

Abdallah El-Zahed, Mahmoud Khalifa, M. El-Zahed, Z. Baka
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Abstract

Green synthesis sources for synthesizing metal oxide nanoparticles are an interesting and expanding research area due to their potential antibacterial applications. Generally, nanoparticles are prepared using different chemical and physical methods that yield toxic or harmful nano-scaled particles in addition to the high cost and complicated processing steps. The present study successfully biosynthesized manganese oxide nanoparticles (MnO NPs) by reducing Manganese sulfate ( MnSO 4 .H 2 O) using the cell-free supernatant of Bacillus subtilis ATCC6633. The formation of MnO NPs was confirmed by UV-Vis spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), Zeta analysis and transmission electron microscope (TEM). The biosynthesized MnO NPs displayed two absorption peaks at 285 and 353 nm. FT-IR spectrum proved the existence of bacterial proteins during the biosynthesis of MnO NPs that might act as stabilizing agents. MnO NPs have a negative charge of -20.4 mV according to Zeta analysis. TEM micrographs showed the rod-shape of MnO NPs with lengths of 70 to 100 nm and diameters of 10 to 23 nm. MnO NPs had a bactericidal action against Bacillus cereus and Escherichia coli with zones of inhibition of 23 and 25 mm, respectively in addition to minimum inhibitory concentration values of 20 and 15 µg/ml, respectively. The obtained results highlighted the possibility of using MnO NPs as a strong antibacterial agent in different industrial and medical applications.
利用枯草芽孢杆菌 ATCC6633 进行抗菌氧化锰纳米粒子的生物合成与表征
由于潜在的抗菌应用,合成金属氧化物纳米粒子的绿色合成源是一个有趣且不断扩展的研究领域。一般来说,纳米粒子的制备采用不同的化学和物理方法,除了成本高、加工步骤复杂之外,还会产生有毒或有害的纳米级粒子。本研究利用枯草芽孢杆菌 ATCC6633 的无细胞上清液还原硫酸锰(MnSO 4 .H 2 O),成功地生物合成了纳米氧化锰颗粒(MnO NPs)。紫外可见光谱、傅立叶变换红外光谱(FT-IR)、Zeta 分析和透射电子显微镜(TEM)证实了 MnO NPs 的形成。生物合成的 MnO NPs 在 285 和 353 纳米波长处显示出两个吸收峰。傅立叶变换红外光谱证明,在生物合成 MnO NPs 的过程中存在细菌蛋白质,它们可能起到稳定剂的作用。根据 Zeta 分析,MnO NPs 带有 -20.4 mV 的负电荷。TEM 显微照片显示,MnO NPs 呈棒状,长度为 70 至 100 nm,直径为 10 至 23 nm。MnO NPs 对蜡样芽孢杆菌和大肠杆菌具有杀菌作用,抑菌区分别为 23 毫米和 25 毫米,最小抑菌浓度值分别为 20 微克/毫升和 15 微克/毫升。研究结果表明,氧化锰氮氧化物可作为一种强效抗菌剂用于不同的工业和医疗领域。
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