Kinetic Characterization and Partial Purification of Peroxidase in Eucalyptus F1 Hybrids

Q4 Agricultural and Biological Sciences

Journal of Advanced Zoology Pub Date : 2023-12-14 DOI:10.17762/jaz.v44is7.2761

V. Jadon, Barkha Kamal, Geeta Bhandari, Nupur Joshi, Archna Dhasmana, Sanjay Gupta

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Abstract

A differential pattern of peroxidase (E.C. 1.1.11.7) has been observed in reciprocal F1 hybrids of Eucalyptus (FRI-4 and FRI-5) produced using parental combination of E. tereticornis and E. camaldulensis. The hybrids were characterized for the qualitative, kinetic parameters (total and specific activity, units, Km and Vmax) and partial purification of peroxidase. The qualitative study showed the presence of high molecular weight peroxidase isoforms i.e. 260 KD, 250 KD and 110KD bands. A strong maternal inheritance was observed for 260 KD loci in FRI-5. Kinetic characterization of peroxidase revealed that F1 genotypes had efficient peroxidase system over their parental combination resulting in 8 -10 times more heterotic vigour. The FRI-5 genotypes were found to be having efficient peroxidase system with lowest Km values (0.12mM-0.17mM) and higher Vmax values ranging from 0.07-0.14mM min-1mg prot-1. The study revealed the role of maternal inheritance and was found that better genotypes are produced when superior parents are used as seed parent. The results suggest the efficient use of peroxidase marker for the identification of the better genotypes in forest tree species.

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桉树 F1 代杂交种过氧化物酶的动力学特征和部分纯化

在使用 E. tereticornis 和 E. camaldulensis 的亲本组合生产的桉树互交 F1 杂交种（FRI-4 和 FRI-5）中，观察到过氧化物酶（E.C. 1.1.11.7）的不同模式。对杂交种进行了过氧化物酶的定性、动力学参数（总活性和特定活性、单位、Km 和 Vmax）和部分纯化。定性研究显示存在高分子量过氧化物酶同工型，即 260 KD、250 KD 和 110KD 带。在 FRI-5 中，观察到 260 KD 位点具有很强的母系遗传性。过氧化物酶的动力学特征显示，F1 基因型的过氧化物酶系统比亲本组合更有效，从而使杂交活力提高了 8-10 倍。发现 FRI-5 基因型具有高效的过氧化物酶系统，其 Km 值最低（0.12mM-0.17mM），Vmax 值较高，为 0.07-0.14mM min-1mg prot-1。该研究揭示了母系遗传的作用，并发现当使用优良亲本作为种子亲本时，能培育出更好的基因型。研究结果表明，利用过氧化物酶标记物可以有效鉴定林木物种的优良基因型。

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来源期刊

Journal of Advanced Zoology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： The Journal of Advanced Zoology started in 1980 is a peer reviewed half yearly online and prints journal, issued in June and December devoted to the publication of original research work in the various disciplines of Zoology.