Biopolymer Microencapsulation of Bacillus thuringiensis israelensis Tested against Two Populations of Aedes aegypti Larvae

IF 0.3 4区农林科学 Q4 ENTOMOLOGY

Southwestern Entomologist Pub Date : 2023-12-14 DOI:10.3958/059.048.0409

Ariadna Rodríguez-Castro, Guadalupe del Carmen-Reyes Solís, M. G. Maldonado-Blanco, Ma. Guadalupe Rojas-Verde

{"title":"Biopolymer Microencapsulation of Bacillus thuringiensis israelensis Tested against Two Populations of Aedes aegypti Larvae","authors":"Ariadna Rodríguez-Castro, Guadalupe del Carmen-Reyes Solís, M. G. Maldonado-Blanco, Ma. Guadalupe Rojas-Verde","doi":"10.3958/059.048.0409","DOIUrl":null,"url":null,"abstract":"Abstract. Aedes aegypti Linnaeus is a vector of viral diseases such as yellow fever, classical and haemorrhagic Dengue fever, Chikungunya, and Zika fever that was declared an international health emergency by the WHO in the summer of 2016. An alternative for larval control is use of microbial insecticides based on Bacillus thuringiensis var. israelensis (Bti). In this work were prepared three formulations based on this bacterium using biodegradable polymers such as pectin, acacia gum, and mixtures of them tested in a laboratory against two third-instar Ae. aegypti larval populations and compared with a commercial product. The results showed 71-72% of the sensitive New Orleans Aedes aegypti strain were dead the first day post-application, by Bti-acacia gum (F2 Bti-Gac) and Bti-acacia gum-pectin (F3 Bti-G-Pec) formulations, while commercial formulation Bactimos® Briquets (F5 Bac) killed significantly the most (91.18%). For Day 7 post-application, formulations of acacia gum (F2 Bti-Gac) and Bti-acacia gum-pectin (F3 Bti-G-Pec) and Bactimos® Briquets (F5 Bac) killed significantly similar numbers of mosquitoes, about 80%, while the two pectin formulations, recent (F1 Bti-Pec) and old (F4 Bti-Pec-Ant), killed only 33-34%. For the application test of the formulations on the Juan Pablo Lirios larval population (from urban sites), formulations of recent pectin (F1 Bti-Pec) and from years ago (F4 Bti-Pec-Ant) did not show any effect on mortality of A. aegypti larvae on the 1st day after application, opposite formulation with acacia gum (F2 Bti-Gac) that killed most, about 50% the first day post-application, while Bactimos® Briquets killed 37%. However, numbers killed decreased notably the following sampling days for both Bti formulations and commercial product. Resumen. Aedes aegypti Linnaeus (Díptera: Culicidae) es un vector de enfermedades virales como la fiebre amarilla, Dengue clásico y Dengue hemorrágico, Chikungunya, y Zika, la cual fue declarada en el verano del 2016 como una emergencia de salud internacional por la OMS. Una alternativa para control larval es el uso de insecticidas microbianos a base de Bacillus thuringiensis var. Israelensis (Bti). En este trabajo se prepararon 3 formulaciones a base de esta bacteria, usando polímeros tales como pectina, goma acacia y mezclas de estos, los cuales fueron probados en laboratorio contra dos poblaciones larvales de Aedes aegypti de tercer estadío tardío y comparadas con un producto comercial. Los resultados encontrados mostraron que la cepa sensitiva New Orleans de A. aegypti, el primer día postaplicación presentó mortalidad de 71-72% causada por las formulaciones Bti-goma acacia y Bti-goma acacia-pectina, mientras que el Bactimos® Briquets causó alrededor de 90% de mortalidad. Para el día 7 postaplicación, tanto las formulaciones de Bti-goma acacia, Bti-goma acacia-pectina y Bactimos® Briquets mostraron significativamente similar mortalidad, cercana al 80%, en tanto las formulaciones de pectina reciente y antigua presentaron menor mortalidad de 34%. En la prueba de aplicación de formulaciones contra Aedes aegypti colectada de sitios urbanos (Juan Pablo Lirios), la formulación de Bti-goma acacia presentó la mayor mortalidad el día 1 postaplicación, cercana al 50%, mientras que el Bactimos® Briquets causó 37% de mortalidad. Sin embargo, la mortalidad disminuyó notablemente para los siguientes días tanto para las formulaciones de Bti aplicadas como para el producto comercial.","PeriodicalId":21930,"journal":{"name":"Southwestern Entomologist","volume":"90 1","pages":"869 - 878"},"PeriodicalIF":0.3000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Southwestern Entomologist","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3958/059.048.0409","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENTOMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract. Aedes aegypti Linnaeus is a vector of viral diseases such as yellow fever, classical and haemorrhagic Dengue fever, Chikungunya, and Zika fever that was declared an international health emergency by the WHO in the summer of 2016. An alternative for larval control is use of microbial insecticides based on Bacillus thuringiensis var. israelensis (Bti). In this work were prepared three formulations based on this bacterium using biodegradable polymers such as pectin, acacia gum, and mixtures of them tested in a laboratory against two third-instar Ae. aegypti larval populations and compared with a commercial product. The results showed 71-72% of the sensitive New Orleans Aedes aegypti strain were dead the first day post-application, by Bti-acacia gum (F2 Bti-Gac) and Bti-acacia gum-pectin (F3 Bti-G-Pec) formulations, while commercial formulation Bactimos® Briquets (F5 Bac) killed significantly the most (91.18%). For Day 7 post-application, formulations of acacia gum (F2 Bti-Gac) and Bti-acacia gum-pectin (F3 Bti-G-Pec) and Bactimos® Briquets (F5 Bac) killed significantly similar numbers of mosquitoes, about 80%, while the two pectin formulations, recent (F1 Bti-Pec) and old (F4 Bti-Pec-Ant), killed only 33-34%. For the application test of the formulations on the Juan Pablo Lirios larval population (from urban sites), formulations of recent pectin (F1 Bti-Pec) and from years ago (F4 Bti-Pec-Ant) did not show any effect on mortality of A. aegypti larvae on the 1st day after application, opposite formulation with acacia gum (F2 Bti-Gac) that killed most, about 50% the first day post-application, while Bactimos® Briquets killed 37%. However, numbers killed decreased notably the following sampling days for both Bti formulations and commercial product. Resumen. Aedes aegypti Linnaeus (Díptera: Culicidae) es un vector de enfermedades virales como la fiebre amarilla, Dengue clásico y Dengue hemorrágico, Chikungunya, y Zika, la cual fue declarada en el verano del 2016 como una emergencia de salud internacional por la OMS. Una alternativa para control larval es el uso de insecticidas microbianos a base de Bacillus thuringiensis var. Israelensis (Bti). En este trabajo se prepararon 3 formulaciones a base de esta bacteria, usando polímeros tales como pectina, goma acacia y mezclas de estos, los cuales fueron probados en laboratorio contra dos poblaciones larvales de Aedes aegypti de tercer estadío tardío y comparadas con un producto comercial. Los resultados encontrados mostraron que la cepa sensitiva New Orleans de A. aegypti, el primer día postaplicación presentó mortalidad de 71-72% causada por las formulaciones Bti-goma acacia y Bti-goma acacia-pectina, mientras que el Bactimos® Briquets causó alrededor de 90% de mortalidad. Para el día 7 postaplicación, tanto las formulaciones de Bti-goma acacia, Bti-goma acacia-pectina y Bactimos® Briquets mostraron significativamente similar mortalidad, cercana al 80%, en tanto las formulaciones de pectina reciente y antigua presentaron menor mortalidad de 34%. En la prueba de aplicación de formulaciones contra Aedes aegypti colectada de sitios urbanos (Juan Pablo Lirios), la formulación de Bti-goma acacia presentó la mayor mortalidad el día 1 postaplicación, cercana al 50%, mientras que el Bactimos® Briquets causó 37% de mortalidad. Sin embargo, la mortalidad disminuyó notablemente para los siguientes días tanto para las formulaciones de Bti aplicadas como para el producto comercial.

查看原文本刊更多论文

针对两种埃及伊蚊幼虫种群的苏云金芽孢杆菌生物聚合物微胶囊试验

摘要埃及伊蚊（Aedes aegypti Linnaeus）是黄热病、典型和出血性登革热、基孔肯雅热和寨卡热等病毒性疾病的传播媒介，2016 年夏天，世界卫生组织宣布埃及伊蚊为国际紧急卫生状况。控制幼虫的另一种方法是使用基于苏云金芽孢杆菌变种（Bti）的微生物杀虫剂。在这项工作中，使用果胶、刺槐胶等生物可降解聚合物以及它们的混合物制备了三种基于这种细菌的制剂，在实验室中对两种埃及蚁三龄幼虫种群进行了测试，并与一种商业产品进行了比较。结果表明，Bti-刺槐胶（F2 Bti-Gac）和 Bti-刺槐胶-pectin（F3 Bti-G-Pec）制剂在施用后第一天杀死了 71-72% 的敏感的新奥尔良埃及伊蚊，而商业制剂 Bactimos® Briquets（F5 Bac）的致死率最高（91.18%）。施药后第 7 天，刺槐胶（F2 Bti-Gac）和 Bti-刺槐胶-pectin（F3 Bti-G-Pec）配方和 Bactimos® Briquets（F5 Bac）配方杀灭的蚊子数量相当，约为 80%，而两种果胶配方，即新配方（F1 Bti-Pec）和旧配方（F4 Bti-Pec-Ant），只杀灭了 33-34%的蚊子。在对胡安-巴勃罗-利里奥斯幼虫群（来自城市地区）的配方施用试验中，新果胶配方（F1 Bti-Pec）和多年前的果胶配方（F4 Bti-Pec-Ant）在施用后第一天对埃及蝇幼虫的死亡率没有任何影响，与之相反，含有刺槐胶的配方（F2 Bti-Gac）杀灭的埃及蝇幼虫最多，施用后第一天杀灭率约为 50%，而 Bactimos® Briquets 杀灭率为 37%。然而，在接下来的取样天数中，Bti 配方和商用产品的杀灭数量都明显下降。摘要。埃及伊蚊（Díptera: Culicidae）是一种病毒性疾病的传播媒介，如痢疾、登革热、血吸虫病、基孔肯雅病毒和寨卡病毒。控制幼虫的一种替代方法是使用基于苏云金芽孢杆菌以色列变种（Bti）的杀虫剂。在这项研究中，研究人员使用果胶、金合欢和混合剂等聚合物制备了 3 种以这种细菌为基础的制剂，在实验室中对埃及伊蚊的两个幼虫群进行了试验，并与一种商业产品进行了比较。发现的结果表明，埃及伊蚊对新奥尔良牛肝菌敏感，在使用后的第一天，Bti-goma 刺槐和 Bti-goma 刺槐pectina 配方造成的死亡率为 71-72%，而 Bactimos® Briquets 造成的死亡率约为 90%。在施用后第 7 天，Bti-goma 刺槐、Bti-goma 刺槐pectina 和 Bactimos® Briquets 的死亡率非常接近，接近 80%，而 Bactimos® Briquets 的死亡率较低，仅为 34%。在针对城市（胡安-巴勃罗-利里奥斯）收集的埃及伊蚊的配方应用试验中，Bti-goma acacia 配方在应用后第 1 天造成的死亡率最高，接近 50%，而 Bactimos® Briquets 造成的死亡率为 37%。尽管如此，在随后的几天里，Bti 应用配方和商业产品的死亡率都明显下降。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Southwestern Entomologist 生物-昆虫学

CiteScore

0.60

自引率

25.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Manuscripts submitted for consideration for publication in the Southwestern Entomologist should report results of entomological research in the southwestern United States or Mexico or should report results of studies on entomological species, relevant to this region, which may be done elsewhere, provided such results are geographically applicable. Manuscripts that report results of routine laboratory or field experiments for which the primary purpose is gathering baseline data or those that report results of a continuous evaluation program such as preliminary pesticide evaluation experiments, species lists with no supporting biological data, or preliminary plant resistance evaluations are not acceptable. However, reports of experiments with insecticides, acaricides, and microbials are acceptable if they are comprehensive and include data related to economics, resistance, toxicology, or other broad subject areas. Bibliographies will not be published in Southwestern Entomologist.