Analytical and bio-analytical methods of rofecoxib: A comprehensive review

Abstract

A selective COX-2 inhibitor Rofecoxib it is a non-steroidal anti-inflammatory (NSAIDs) medicines work by inhibiting the COX enzyme, which is a mediator of inflammation. Rofecoxib is used to treat rheumatoid arthritis, osteoarthritis, and primary dysmenorrhea. The main objective of this study is to examine Rofecoxib in pharmaceutical and biological formulations both qualitative and quantitative. In this review paper, we have outlined the approaches based on UV/Vis spectroscopy, High-performance liquid chromatography (HPLC), High-performance thin layer chromatography (HPTLC) and Liquid chromatography-mass spectrometry (LC-MS) for estimating rofecoxib. We have also discussed the bioanalytical methods used to analyse RFX. In conclusion, this review paper will aid researchers in developing new techniques for drug estimation in biological fluids and pharmaceutical dosage forms.