Anti-Aging Properties of Temulawak Extract (Curcuma xanthorrhiza L.) by In Vitro Assay

Folia Medica Indonesiana Pub Date : 2023-12-10 DOI:10.20473/fmi.v59i4.44944

Teresa Liliana Wargasetia, Wahyu Widowati, Anis Syabani Muthmainnah, Rizal

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Abstract

Highlights:1. Elastase, hyaluronidase, and tyrosinase can all be inhibited by temulawak extract.2. Temulawak extract has strong anti-aging ability and can be used as a candidate for cosmetic applications. AbstractThe process of aging is the gradual loss of a tissue's capacity to heal and maintain normal or physiological form and function. Elastase, hyaluronidase, and tyrosinase are the enzymes that contribute to skin aging. The anti-aging effect is connected to the inhibition of these enzymes' activity. Many medicinal plants with active metabolite have been utilized extensively to treat aging. Active compounds of temulawak (Curcuma xanthorrhiza L.) such as phenolic, curcuminoid and xanthorrhizol have potential as antiaging. This study aims to determine whether temulawak extract (TE), a potential candidate for cosmeceutical agent, has anti-aging characteristics by inhibiting the elastase, hyaluronidase, and tyrosinase enzymes. TE was processedxby PT FAST. Stock solution of TE was made by dissolving 20 mg of extract with 1 ml of DMSO 100%, the stock solution was then diluted to achieve the concentrations level 31.25 - 1000 μg/ml working solution of TE. The antiaging activity assays were carried out at PT Aretha Medika Utama. In vitro antiaging assay of TE includes elastase, hyaluronidase, and tyrosinase enzyme inhibition at 7 level of various concentrations of 2.08-66.67 μg/ml for anti-elastase assay, 5.21-166.67 μg/ml for anti-hyaluronidase assay, and 3.125-100 μg/ml for anti-tyrosinase assay with three replications. SPSS software was used to conduct the statistical analysis (p<0,05 is considered significant). TE exhibited highest inhibition percentage of 82.72%, 89.41%, and 94.17% for anti-tyrosinase, anti-elastase, and anti-hyaluronidase, respectively. The median inhibitory concentration (IC50) for elastase inhibitory activity was 10.66 μg/ml, hyaluronidase 70.39 μg/ml, and tyrosinase 55.87 μg/ml. According to this study, TE can be used as cosmeceu

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通过体外实验研究Temulawak（莪术）提取物的抗衰老特性

研究亮点：1.淡竹叶提取物可抑制弹性蛋白酶、透明质酸酶和酪氨酸酶；2.淡竹叶提取物具有很强的抗衰老能力，可作为化妆品的候选原料；3.淡竹叶提取物具有很强的抗衰老能力，可作为化妆品的候选原料；4.淡竹叶提取物具有很强的抗衰老能力，可作为化妆品的候选原料。摘要衰老过程是组织愈合和维持正常或生理形态和功能的能力逐渐丧失的过程。弹性蛋白酶、透明质酸酶和酪氨酸酶是导致皮肤老化的酶类。抗衰老作用与抑制这些酶的活性有关。许多药用植物的活性代谢物已被广泛用于治疗衰老。Temulawak （Curcuma xanthorrhiza L.）的活性化合物，如酚类、姜黄素和黄根酚类，具有抗衰老的潜力。本研究旨在确定作为化妆品潜在候选药剂的 Temulawak 提取物（TE）是否具有通过抑制弹性蛋白酶、透明质酸酶和酪氨酸酶来抗衰老的特性。TE 经 PT FAST 处理。将 20 毫克提取物与 1 毫升 100% 的二甲基亚砜溶解，制成 TE 的储备溶液，然后将储备溶液稀释至浓度为 31.25 - 1000 μg/ml 的 TE 工作溶液。抗衰老活性试验在 PT Aretha Medika Utama 进行。TE 的体外抗衰老试验包括弹性蛋白酶、透明质酸酶和酪氨酸酶的酶抑制作用，7 个不同的浓度水平：抗弹性蛋白酶试验为 2.08-66.67 μg/ml，抗透明质酸酶试验为 5.21-166.67 μg/ml，抗酪氨酸酶试验为 3.125-100 μg/ml，3 次重复。采用 SPSS 软件进行统计分析（P<0.05 为差异显著）。TE 对抗酪氨酸酶、抗弹性蛋白酶和抗透明质酸酶的抑制率最高，分别为 82.72%、89.41% 和 94.17%。弹性蛋白酶抑制活性的中位抑制浓度（IC50）为 10.66 μg/ml，透明质酸酶为 70.39 μg/ml，酪氨酸酶为 55.87 μg/ml。根据这项研究，TE 可用作化妆品。

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Folia Medica Indonesiana

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审稿时长

16 weeks