The Influence of Syzygium aromaticum Ethanolic Extract Against Streptozotocin-Induced Diabetic Rats

IF 1.1 Q3 CHEMISTRY, MULTIDISCIPLINARY

Egyptian Journal of Chemistry Pub Date : 2023-12-10 DOI:10.21608/ejchem.2023.244595.8772

Amal Hussien, Amal Fyiad, Mamdouh Ali, Abeer Mahmoud

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引用次数: 0

Abstract

Diabetes Mellitus (DM) is characterized by hyperglycemia and increased level of reactive oxygen species (ROS). Our study verified Syzygium aromaticum (clove) ethanolic extract phytochemical composition by high-performance liquid chromatography (HPLC) analysis to assess clove extract possible impact on glucose status, liver and kidney functions, and oxidative stress in diabetic model. Dry Syzygium aromaticum buds obtained from the market, grinded, extracted by 70% ethanol. Thirty two adult male rats were classified into four groups (8per each). Controls (CG), Syzygium aromaticum group (SAG): rats received (500 mg/kg b.wt.) clove ethanolic extract, Diabetic rats (DG): injected with Streptozotocin [STZ, 75 mg/kg b.wt. (a single dose)], intraperitoneally (IP). Diabetic treated group (DSA): rats injected with STZ as in (DG) group and at the eighth day rats were treated with the ethanolic extract of clove as in (SAG) group. Animals were sacrificed after 14 days. Glucose level, liver enzymes, amylase activity, kidney function tests, total antioxidant capacity (TAC), malondialdehyde (MDA), albumin and total protein (TP) levels were measured. Also, the body weights of each rat were determined throughout the experimental period. Administration of STZ increased glucose level, hepatic markers, urea, creatinine, and MDA levels. Conversely, serum TAC, albumin, TP and α-amylase activity were significantly decreased. All the aforementioned parameters were improved due to the treatment of diabetic rats with clove ethanolic extract. Clove extract could be a promising therapeutic agent in the treatment of diabetic models.

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芳香茜草乙醇提取物对链脲佐菌素诱导的糖尿病大鼠的影响

糖尿病（DM）的特点是高血糖和活性氧（ROS）水平升高。我们的研究通过高效液相色谱法（HPLC）分析验证了丁香乙醇提取物的植物化学成分，以评估丁香提取物对糖尿病模型的血糖状况、肝肾功能和氧化应激可能产生的影响。丁香是从市场上购买的干丁香芽，研磨后用 70% 的乙醇提取。将 32 只成年雄性大鼠分为四组（每组 8 只）。对照组 (CG)；丁香组 (SAG)：大鼠接受（500 毫克/千克体重）丁香乙醇提取物；糖尿病大鼠 (DG)：腹腔注射（IP）链脲佐菌素[STZ，75 毫克/千克体重（单剂量）]。糖尿病治疗组（DSA）：与（DG）组大鼠一样注射 STZ，第八天用丁香乙醇提取物治疗（SAG）组大鼠。14 天后动物被处死。测量葡萄糖水平、肝酶、淀粉酶活性、肾功能测试、总抗氧化能力（TAC）、丙二醛（MDA）、白蛋白和总蛋白（TP）水平。此外，在整个实验期间还测定了每只大鼠的体重。给予 STZ 会增加葡萄糖水平、肝脏指标、尿素、肌酐和 MDA 水平。相反，血清 TAC、白蛋白、TP 和 α 淀粉酶活性则明显下降。用丁香乙醇提取物治疗糖尿病大鼠后，上述所有指标都得到了改善。丁香提取物是一种治疗糖尿病模型的有效药物。

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来源期刊

Egyptian Journal of Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

2.40

自引率

23.10%

发文量

977

期刊介绍： The Egyptian Journal of Chemistry is published bimonthly by the Egyptian Chemical Society. Contributions from fields of pure chemistry are invited. The fields includes general and physical chemistry, analytical and inorganic chemistry, organic and biological chemistry, and applied and materials chemistry.