Lymphocyte Cell Expressions and SARS-COV-2 Antibody Titers in Healthy Donors’ Blood at an Indonesian Blood Transfusion Center (UTD PMI Surabaya)

Folia Medica Indonesiana Pub Date : 2023-12-10 DOI:10.20473/fmi.v59i4.38954

Adelia Gita Prasasti, Evy Diah Woelansari, Anita Dwi Suhariyadi, Anggraini

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Abstract

Highlights: 1. There was no relationship between total lymphocyte count and antibody titers against SARS-CoV-2.2. This original research report offers information on the several blood test result after getting COVID-19vaccines and provides data that the public may use as scientific evidence to the further research. Abstract SARS-CoV-2 is a virus that infects the respiratory tract and attacks the respiratory mucosa and epithelial cells.Lymphocytes are a subset of agranulocyte leukocytes that have a role in the immune response to pathogenicmicroorganisms' attacks. The number of lymphocytes will increase if a virus causes an infection. An antibody is aprotective protein produced by the body's immune system in response to the presence of an antigen. Antibodies bind and inactivate foreign substances so that the replication of pathogens can be prevented and destroyed. This mechanism can be expressed through the total number of lymphocyte cells. The objective of this study was to define the relationship between the number of lymphocytes and antibody titers against SARS-CoV-2. The type of research used was analytic observational with a cross-sectional approach using quantitative analysis methods and a purposive sampling method for sampling. The samples used were 30 donor blood samples from UTD PMI Surabaya. This research was conducted in May 2022 at the Hematology Laboratory, Department of Medical Laboratory Technology, Poltekkes, Ministry of Health, Surabaya, to examine the number of lymphocyte cells using the flowcytometry method and the Immunoserology Laboratory of the Surabaya Health Laboratory Center for antibody titer examination using the ELISA (Enzyme-Linked Immuno Sorbent Assay) method. The study showed an average lymphocyte level of 2.2633 x 103/µl and an average antibody titer value expressed in OD of 0.2197. Data analysis using Spearman's rank correlation statistical test revealed p = 0.262 0.005. It showed no relationship between total lymphocyte count and antibody titers against SARS-CoV-2

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印度尼西亚输血中心（UTD PMI Surabaya）健康献血者血液中的淋巴细胞表达和 SARS-COV-2 抗体滴度

要点：1.总淋巴细胞数与 SARS-CoV-2 抗体滴度之间没有关系。2. 本原创研究报告提供了有关接种 COVID-19 疫苗后几次血液检测结果的信息，并为公众提供了数据，可作为进一步研究的科学依据。摘要 SARS-CoV-2 是一种感染呼吸道并攻击呼吸道粘膜和上皮细胞的病毒。淋巴细胞是粒细胞白细胞的一个亚群，在对病原微生物攻击的免疫反应中发挥作用。如果病毒引起感染，淋巴细胞的数量就会增加。抗体是人体免疫系统针对抗原的存在而产生的保护性蛋白质。抗体能结合外来物质并使其失活，从而阻止和消灭病原体的复制。这种机制可以通过淋巴细胞的总数来体现。本研究的目的是确定淋巴细胞数量与 SARS-CoV-2 抗体滴度之间的关系。研究采用的是横断面分析观察法，使用定量分析方法和目的取样法进行取样。使用的样本是来自泗水UTD PMI的30份献血者血液样本。这项研究于2022年5月在泗水卫生部Poltekkes医学实验室技术部血液学实验室进行，采用流式细胞计数法检测淋巴细胞数量，并在泗水卫生实验室中心免疫血清学实验室采用ELISA（酶联免疫吸附试验）法检测抗体滴度。研究结果显示，淋巴细胞平均水平为 2.2633 x 103/µl，以 OD 表示的抗体滴度平均值为 0.2197。使用斯皮尔曼秩相关统计检验法进行的数据分析显示，P = 0.262 0.005。结果表明，淋巴细胞总数与 SARS-CoV-2 抗体滴度之间没有关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Folia Medica Indonesiana

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审稿时长

16 weeks