Exploring polyphenols extraction from Thuja occidentalis L. bark: Optimization, phytochemical profiling, and biological evaluation

IF 1.3 4区农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD

Bioresources Pub Date : 2023-12-11 DOI:10.15376/biores.19.1.842-857

N. Coman, Mihai Babotă, Ingrid Nădășan, Rideg Lucian, Ruxandra Ștefănescu, Oleg Frumuzachi, A. Mocan, Alexandru Nicolescu, C. Tanase

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引用次数: 0

Abstract

Polyphenols were extracted from Thuja occidentalis L. bark and characterized in terms of phytochemical composition and biological activity. The optimization process investigated the impacts of extraction duration, ultrasound amplitude, and ethanol concentration on the total phenolic content in the extract. The antioxidant capacity was examined using DPPH and ABTS assays, and anti-enzyme activity against alpha-glucosidase, alpha-amylase, lipase, acetylcholinesterase, and tyrosinase was determined. The experimental model revealed optimal extraction parameters: a hydroethanolic solvent with 44% v:v ethanol, a 15-minute extraction time, and a 40% ultrasound amplitude. These parameters were validated and confirmed by reaching the target Total Phenolic Content (TPC) predicted by MODDE software. The resulting lyophilized extract exhibited a high polyphenolic content (161.84 ± 23.48 mg GAE/g dry extract) and demonstrated strong antioxidant properties. Notably, it showed significant inhibition of alpha-glucosidase and alpha-amylase, suggesting potential antidiabetic effects, though its inhibition of tyrosinase activity was relatively weak. These findings provide a foundation for further investigations to confirm the qualitative and quantitative presence of specific polyphenolic metabolites in the extract and elucidate the mechanisms underlying its bioactive properties.

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探索从西洋杉树皮中提取多酚：优化、植物化学成分分析和生物学评价

从西番莲树皮中提取了多酚，并对其植物化学成分和生物活性进行了表征。优化过程研究了萃取时间、超声波振幅和乙醇浓度对萃取物中总酚含量的影响。使用 DPPH 和 ABTS 法检测了抗氧化能力，并测定了对α-葡萄糖苷酶、α-淀粉酶、脂肪酶、乙酰胆碱酯酶和酪氨酸酶的抗酶活性。实验模型揭示了最佳萃取参数：含 44% v:v 乙醇的水乙醇溶剂、15 分钟萃取时间和 40% 超声波振幅。通过 MODDE 软件预测的目标总酚含量（TPC），验证并确认了这些参数。冻干提取物的多酚含量很高（161.84 ± 23.48 mg GAE/g），具有很强的抗氧化性。值得注意的是，它对α-葡萄糖苷酶和α-淀粉酶有明显的抑制作用，这表明它具有潜在的抗糖尿病作用，但对酪氨酸酶活性的抑制作用相对较弱。这些发现为进一步研究提供了基础，以确认提取物中特定多酚代谢物的定性和定量存在，并阐明其生物活性特性的内在机制。

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来源期刊

Bioresources 工程技术-材料科学：纸与木材

CiteScore

2.90

自引率

13.30%

发文量

397

审稿时长

2.3 months

期刊介绍： The purpose of BioResources is to promote scientific discourse and to foster scientific developments related to sustainable manufacture involving lignocellulosic or woody biomass resources, including wood and agricultural residues. BioResources will focus on advances in science and technology. Emphasis will be placed on bioproducts, bioenergy, papermaking technology, wood products, new manufacturing materials, composite structures, and chemicals derived from lignocellulosic biomass.