Effect of a Salvia officinalis and Hypericum perforatum mixture on improving memory and cognitive decline

IF 1.8 Q3 PHARMACOLOGY & PHARMACY

Advances in Traditional Medicine Pub Date : 2023-12-21 DOI:10.1007/s13596-023-00732-z

Jiwon Lee, Shinhui Lee, Woonhee Jo, Hwee Won Ji, Minsu Pyeon, Myungmi Moon, Jihwon Yun, Junho H. Lee, Sung-Oh Sohn

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Abstract

In this study, we explored the effects of herbal mixture containing Sage (Salvia officinalis) and St. John’s Wort (Hypericum perforatum) on cognitive and memory impairment, considering their known cognitive-enhancing properties. We identified ‘rosmarinic acid’ and ‘hyperoside’ as marker components influencing memory in Sage and St. John’s Wort, respectively. Quantification of these marker compounds in the herbal extracts was performed using high-performance liquid chromatography. Using human neuroblastoma SH-SY5Y cell and murine microglia BV-2 cell, we investigated the effects of the herbal mixture on oxidative stress induced by scopolamine and lipopolysaccharide. Cell viability assays, nitric oxide level quantification, and measurement of cytokines (tumor necrosis factor-α, interleukin-1β) and catalase expression were performed. The results indicated positive effects of the herbal mixture on these parameters. Furthermore, the herbal mixture oral administered to mouse and induced intentional inflammatory responses and memory impairment by treating them with scopolamine. Cognitive and memory impairment were evaluated using Morris Water Maze and T-maze tests, while cytokines (TNF-a, IL-1β) and Catalase in mouse serum were quantified. The group receiving the herbal mixture at a concentration of 10 mg/kg demonstrated significantly reduced TNF-a and IL-1β levels, increased Catalase expression, and improved cognitive performance in the Morris Water Maze and T-maze tests. These findings suggest that the intake of the herbal mixture containing Sage (S. officinalis) and St. John’s Wort (H. perforatum) may ameliorate cognitive and memory impairment through anti-inflammatory and antioxidant mechanisms.

Graphical abstract

Sage (Salvia officinalis) and St. John’s wort (Hypericum perforatum) ethanol extract was mixed in a 1:1 ratio, and the effect of improving memory and cognition was confirmed through cell and animal experiments

Abstract Image

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丹参和贯叶连翘混合物对改善记忆力和认知能力下降的影响

考虑到鼠尾草（Salvia officinalis）和圣约翰草（Hypericum perforatum）已知的增强认知能力的特性，我们在本研究中探讨了这两种草药混合物对认知和记忆损伤的影响。我们发现 "迷迭香酸 "和 "金丝桃苷 "分别是鼠尾草和圣约翰草中影响记忆的标记成分。我们使用高效液相色谱法对草药提取物中的这些标记化合物进行了定量。我们使用人神经母细胞瘤 SH-SY5Y 细胞和鼠小胶质细胞 BV-2 研究了草药混合物对东莨菪碱和脂多糖诱导的氧化应激的影响。实验中进行了细胞活力测定、一氧化氮水平定量、细胞因子（肿瘤坏死因子-α、白细胞介素-1β）和过氧化氢酶表达测定。结果表明，草药混合物对这些参数有积极影响。此外，给小鼠口服草药混合物，并用东莨菪碱处理小鼠，诱发有意的炎症反应和记忆损伤。小鼠血清中的细胞因子（TNF-a、IL-1β）和过氧化氢酶被量化。接受 10 毫克/千克浓度草药混合物的小鼠组的 TNF-a 和 IL-1β 水平明显降低，过氧化氢酶表达增加，在莫里斯水迷宫和 T 型迷宫测试中的认知表现也有所改善。这些研究结果表明，摄入含有鼠尾草（Salvia officinalis）和圣约翰草（Hypericum perforatum）的草药混合物可通过抗炎和抗氧化机制改善认知和记忆损伤。

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来源期刊

Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-

CiteScore

4.30

自引率

0.00%

发文量

期刊介绍： Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.