{"title":"Advances in Rolling Circle Amplification (RCA)-Based DNA-Functional Materials for Cancer Diagnosis and Therapy","authors":"Xun You, Jing Wang, Xiaocui Guo, Dayong Yang","doi":"10.1002/anbr.202300108","DOIUrl":null,"url":null,"abstract":"<p>Developing biocompatible material systems with accurate functional designability and powerful integration capability is the urgent demand of efficient cancer diagnosis and therapy. Deoxyribonucleic acids (DNAs) as biomacromolecules are characterized with sequence programmability, rich biological activity, and molecular recognition, and show great performance in the fabrication of biomedical materials. Rolling circle amplification (RCA) is an efficient isothermal enzymatic amplification strategy for production of ultralong single-stranded DNA (ssDNA) with defined repeat sequences and structures. By virtue of rational design of the RCA templates sequences, the produced ssDNA enables to integrate and amplify the required function modules, which endows RCA-based DNA materials with extraordinary performance in cancer therapeutics. In this review, RCA-based strategies for integration of functional modules are systematically summarized; construction of RCA-based functional DNA materials and their recent progress in cancer therapeutics including detection, bioimaging, and therapy are overviewed; and finally the opportunities and challenges of RCA-based assembly strategy in terms of material construction and applications in cancer diagnosis and therapy are discussed. It is envisioned that RCA-based DNA-functional materials will provide typical paradigms for the application of DNA-functional materials in the field of cancer therapeutics, and hopefully provide more possibilities for precision medicine.</p>","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 3","pages":""},"PeriodicalIF":4.0000,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300108","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Nanobiomed Research","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/anbr.202300108","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Developing biocompatible material systems with accurate functional designability and powerful integration capability is the urgent demand of efficient cancer diagnosis and therapy. Deoxyribonucleic acids (DNAs) as biomacromolecules are characterized with sequence programmability, rich biological activity, and molecular recognition, and show great performance in the fabrication of biomedical materials. Rolling circle amplification (RCA) is an efficient isothermal enzymatic amplification strategy for production of ultralong single-stranded DNA (ssDNA) with defined repeat sequences and structures. By virtue of rational design of the RCA templates sequences, the produced ssDNA enables to integrate and amplify the required function modules, which endows RCA-based DNA materials with extraordinary performance in cancer therapeutics. In this review, RCA-based strategies for integration of functional modules are systematically summarized; construction of RCA-based functional DNA materials and their recent progress in cancer therapeutics including detection, bioimaging, and therapy are overviewed; and finally the opportunities and challenges of RCA-based assembly strategy in terms of material construction and applications in cancer diagnosis and therapy are discussed. It is envisioned that RCA-based DNA-functional materials will provide typical paradigms for the application of DNA-functional materials in the field of cancer therapeutics, and hopefully provide more possibilities for precision medicine.
期刊介绍：
Advanced NanoBiomed Research will provide an Open Access home for cutting-edge nanomedicine, bioengineering and biomaterials research aimed at improving human health. The journal will capture a broad spectrum of research from increasingly multi- and interdisciplinary fields of the traditional areas of biomedicine, bioengineering and health-related materials science as well as precision and personalized medicine, drug delivery, and artificial intelligence-driven health science.
The scope of Advanced NanoBiomed Research will cover the following key subject areas:
▪ Nanomedicine and nanotechnology, with applications in drug and gene delivery, diagnostics, theranostics, photothermal and photodynamic therapy and multimodal imaging.
▪ Biomaterials, including hydrogels, 2D materials, biopolymers, composites, biodegradable materials, biohybrids and biomimetics (such as artificial cells, exosomes and extracellular vesicles), as well as all organic and inorganic materials for biomedical applications.
▪ Biointerfaces, such as anti-microbial surfaces and coatings, as well as interfaces for cellular engineering, immunoengineering and 3D cell culture.
▪ Biofabrication including (bio)inks and technologies, towards generation of functional tissues and organs.
▪ Tissue engineering and regenerative medicine, including scaffolds and scaffold-free approaches, for bone, ligament, muscle, skin, neural, cardiac tissue engineering and tissue vascularization.
▪ Devices for healthcare applications, disease modelling and treatment, such as diagnostics, lab-on-a-chip, organs-on-a-chip, bioMEMS, bioelectronics, wearables, actuators, soft robotics, and intelligent drug delivery systems.
with a strong focus on applications of these fields, from bench-to-bedside, for treatment of all diseases and disorders, such as infectious, autoimmune, cardiovascular and metabolic diseases, neurological disorders and cancer; including pharmacology and toxicology studies.
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