The occurrence of cytokinins and their biosynthesis pathways in epithelioma papulosum cyprini cells

IF 1.1 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Canadian Journal of Chemistry Pub Date : 2023-12-21 DOI:10.1139/cjc-2023-0108

Angela Schincaglia, M. Seegobin, Craig R. Brunetti, R. J. N. Emery

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Abstract

Cytokinins are small signaling molecules present in all kingdoms of life; however, their biological relevance outside of plants is poorly characterized. Cytokinin detection across kingdoms has been shown to be inconsistent and is often organism or cell line dependent. To ensure optimal detection in epithelioma papulosum cyprini (EPC), a cold-blooded vertebrate cell line, a range of biomasses were evaluated. Cytokinins were extracted by solid-phase extraction and then detected using high performance liquid chromatography and tandem mass spectrometry. The objectives were to establish endogenous CK profiles and characterize their biosynthesis in EPC cells. Among the fourteen CKs that were scanned for, only four forms were detected overall within the samples analyzed, including, N6-(Δ2-isopentenyl) adenine-9-riboside (iPR), N6-(Δ2-isopentenyl) adenine-9-riboside-5’ (mono/di/tri)phosphate (iPNT), and cis-zeatin-9-riboside-5’ (mono/di/tri)phosphate (cZNT) and 2‐methylthio‐zeatin (2MeSZ). The total cytokinin concentrations ranged from 0.03 pmol/g to 681.025 pmol/gfwt. The results from this study provided evidence that EPC cells can produce and process cytokinins.

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上皮乳头状瘤细胞中细胞分裂素的出现及其生物合成途径

细胞分裂素是一种存在于所有生物界的小信号分子；然而，它们在植物之外的生物学相关性却鲜为人知。细胞分裂素在不同生物界的检测结果并不一致，通常取决于生物体或细胞系。为确保在冷血脊椎动物细胞系上皮乳头状瘤细胞系（EPC）中进行最佳检测，对一系列生物质进行了评估。采用固相萃取法提取细胞分裂素，然后使用高效液相色谱法和串联质谱法进行检测。目的是建立内源性细胞分裂素谱系，并确定其在 EPC 细胞中的生物合成特征。在扫描的 14 种 CK 中，仅在分析的样本中检测到四种形式，包括 N6-（Δ2-异戊烯基）腺嘌呤-9-核苷（IPR）、N6-（Δ2-异戊烯基）腺嘌呤-9-核苷-5'（单/双/三）磷酸酯（iPNT）、顺式玉米素-9-核苷-5'（单/双/三）磷酸酯（cZNT）和 2-甲硫基玉米素（2MeSZ）。细胞分裂素的总浓度范围为 0.03 pmol/g 至 681.025 pmol/gfwt。这项研究的结果提供了EPC细胞能够产生和处理细胞分裂素的证据。

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来源期刊

Canadian Journal of Chemistry 化学-化学综合

CiteScore

1.90

自引率

9.10%

发文量

审稿时长

1 months

期刊介绍： Published since 1929, the Canadian Journal of Chemistry reports current research findings in all branches of chemistry. It includes the traditional areas of analytical, inorganic, organic, and physical-theoretical chemistry and newer interdisciplinary areas such as materials science, spectroscopy, chemical physics, and biological, medicinal and environmental chemistry. Articles describing original research are welcomed.